JEOL Resourceshttps://www.jeolusa.com/RESOURCES/Analytical-Instruments/Documents-Downloads<sup>14</sup>N decoupled <sup>1</sup>H–<sup>19</sup>F HOESY with ROYALPROBE™ HFX_NM190012Ehttps://www.jeolusa.com/RESOURCES/Analytical-Instruments/Documents-Downloads/14n-decoupled-1h19f-hoesy-with-royalprobe-hfxnm190012eNuclear Magnetic Resonance (NMR)Mon, 10 Feb 2020 20:46:45 GMTFluorine and nitrogen containing organic compounds are often found in a variety of fields such as pharmaceutical and material sciences. Such compounds show unique properties based on their conformations and orientations, which are often induced by hydrogen bonds between N-H and F atoms. Therefore, analysis of molecular conformations and inter-molecular interactions are important. Here, we report 14N decoupled 1H–19F HOESY (hetero nuclear NOE) for fluroinated benzanilide (1) as a demonstration.<h3>Measurements of <sup>14</sup>N decoupling <sup>1</sup>H–<sup>19</sup>F HOESY</h3> <p>It often happens that <sup>1</sup>H NMR analysis is prevented with the severe line broadening of <sup>1</sup>H signal that is bound to <sup>14</sup>N atom. In such a case, the <sup>14</sup>N decoupling technique is useful. <sup>1</sup>H–<sup>19</sup>F 1D-HOESY spectra <em>with</em> and <em>without</em> <sup>14</sup>N decoupling are shown in Fig. 1. Selective excitation was done at F5 (Fig. 1:upper) and F6 (Fig. 1:bottom), respectively. The amide <sup>1</sup>H signal intensity in 1D-HOESY is increased by twice, compared to that measured without <sup>14</sup>N decoupling. Since NOE measurements have general difficulty in low sensitivity, the <sup>14</sup>N decoupling technique is useful for observation of cross peaks with <sup>1</sup>H–<sup>14</sup>N signals. 2D-HOESY with <sup>14</sup>N decoupling measurement is also available (Fig. 2).</p>