JEOL Resources

Food Analysis Solutions

Mon, 27 Jul 2020 21:08:18 GMT

JEOL offers numerous analytical tools to support both "food safety & security" as well as various evaluations of primary, secondary and tertiary functions of foodstuffs which are useful for a wide range of users associated with this field.

This Foodnote introduces the features of each of the instruments and actual analysis examples, and is designed for researchers and engineers who are considering purchases of instruments. This brochure also presents comprehensive evaluations and analysis solutions that can be achieved with combinations of multiple instruments.

Using Solid Phase Microextraction with AccuTOF-DART for Fragrance Analysis

Wed, 04 Mar 2020 15:51:29 GMT

Experimental

A Supelco DVB/Carboxen/PDMS StableFlex SPME fiber was placed in an enclosed plastic bag with a banana for 10 minutes during each analysis. For direct analysis of the SPME fiber, the JEOL AccuTOF-DART™ system was set to the following parameters: needle voltage 3500V, discharge electrode 150V, grid electrode 250V, helium temperature 200 degrees C, and helium flowrate 2.3 L/min. A JEOL GC-Mate II high resolution sector bench top system equipped with a DB5-HT (0.25mm × 30m) was used for the GC-MS portion of the analysis. The GC-Mate II was set to the following parameters: inlet temperature 250 degrees C, split ratio 30, and helium flowrate 1.2 mL/min. The GC oven was set for the following temperature profile: 40 degrees C held for 2 min, ramp from 40 to 260 degrees C at 20 degrees C/min, 260 degrees C held for 2 min.

Rapid screening of stobilurins in crude solid materials (wheat grains) using DART-TOFMS

Wed, 04 Mar 2020 15:48:39 GMT

Direct control of solid materials for pesticide residues is a challenging task enabling fast contamination screening. In our study, we investigated direct analysis of strobilurin fungicides in milled wheat grains. Strobilurins, systemic pesticides originated from natural fungicidal derivatives, play an important role in control of various plant pathogens. Because of their unique protective properties, significant yield enhancements and longer retention of green leaf tissue, strobilurins have been widely used in agriculture since their introduction on the market in 1992. As other pesticides, these compounds are involved in control and monitoring surveys underta

Rapid Detection of Melamine in Dry Milk Using AccuTOF-DART

Wed, 04 Mar 2020 15:37:23 GMT

Experimental

Solid melamine granules were artificially spiked into commercially available dry nonfat milk at levels between 1000ppm and 500 ppb. These samples were then pulverized with a mortar and pestle to homogenize the mixtures. For analysis, the AccuTOF-DART system was set to the following parameters: needle voltage 3500V, discharge electrode 150V, grid electrode 40V, Helium temperature 150 degrees C, and He flowrate 2.3 L/min. A melting point tube was dipped and swirled through the melamine/milk mixture and then placed in the Helium stream between the DART and the AccuTOF atmospheric pressure interface. The data was collected in a matter of seconds from the moment the samples were introduced into the DART stream. A representative mass spectrum is shown in Figure 1 that shows the high resolution and isotopic data for the melamine [M+H]+. Additionally, a semi-quantitative calibration curve was constructed to show the ability of the AccuTOF-DART to measure melamine in dry milk over a dynamic range of concentrations (Figure 2). Furthermore, using this methodology, the AccuTOF-DART was able to detect 1 ppm of melamine in dry milk, which is below the United States Food and Drug Administration’s maximum allowable concentration of 2.5 ppm.

Rapid Detection of Fungicide in Orange Peel

Wed, 04 Mar 2020 15:34:14 GMT

Thiabendazole is an anthelmintic and a highly persistent systematic benzimidazole fungicide that is widely used for controlling spoilage in citrus fruit. It is considered a General Use Pesticide (GUP) in EPA Toxicity Class III – Slight Toxicity. A small piece of orange peel (a few square millimeters in size) from a Florida orange was placed in the DART sampling region. Compounds present in the peel were detected within seconds. Among these were the familiar orange-oil flavor components such as limonene and sinensal as well as polymethoxylated flavones that are attributed with antioxidant and cholesterol-reducing properties.

“No-prep” Analysis of Lipids in Cooking Oils and Detection of Adulterated Olive Oil

Wed, 04 Mar 2020 14:44:28 GMT

Dietary fats are categorized according to the level of unsaturation. Oils are a mixture of triglycerides and free fatty acids. Olive oil contains a high concentration of monounsaturated fatty acids, while other oils such as Canola and safflower oil contain larger amounts of polyunsaturated fatty acids. Characterizing the type of lipids present is important for quality control and for detecting adulteration of more expensive oils (e.g. olive oil) with cheaper products. Analysis by HPLC is time consuming and requires solvents and consumables. DART provides a convenient alternative: no solvents are required and the analysis can be completed in seconds.

Instantaneous Detection of Opiates in Single Poppy Seeds

Wed, 04 Mar 2020 14:31:00 GMT

Poppy seed is a common flavoring ingredient that is known to contain small amounts of opiates. Maximum morphine and codeine concentrations are estimated to be about 33 and 14 micrograms respectively per gram of seed. Consumption of typical amounts of baked goods containing poppy seeds has not been shown to cause any ill effects. However, ingestion of poppy seeds may result in false positives from drug tests. Single poppy seeds from different sources were analyzed independently in two different laboratories by using the DART™/AccuTOF™ combination. The resulting mass spectra were nearly identical.

Distribution of Capsaicin in Chili Peppers

Wed, 04 Mar 2020 10:28:56 GMT

We examined different parts of a hot pepper to determine which part of the pepper contains the highest concentration of capsaicin. Different sections of the pepper were placed between the DART and the AccuTOF orifice. Little capsaicin was found in the fleshy part of the pepper; higher concentrations were found in the pepper seeds. The highest concentration of capsaicin was found in the membrane inside the pepper pod onto which the seeds are attached.

Direct analysis of caffeine in soft drinks and coffee and tea infusions

Wed, 04 Mar 2020 10:13:57 GMT

Caffeine (Figure 1), a xanthine alkaloid acting as psychoactive stimulant and mild diuretic in human, is an integral part of diet of many people. It is often found in natural products such as tea, coffee and cocoa beans, cola nuts and many others. Analysis of caffeine in various foods and beverages is an important task for analytical laboratories, as its content is considered in assessment of product quality (coffee, cocoa beans and tea). Due to its physiological effect, the amount of caffeine is regulated in selected foods in EU. Maximum limits are set for some soft drinks to which caffeine is added. HPLC methods employing UV detection are commonly used for its control. While for soft drinks and coffee/tea infusions, the sample preparation is not too much time demanding, LC separation of sample components becomes a limiting step in laboratory throughput. Employing AccuTOF-DART system offers straightforward examination of caffeine content in tens of samples per hour, thanks to omitting separation step. Isotope dilution is used for target analyte quantification.

Detection of Unstable Compound Released by Chopped Chives

Sun, 23 Feb 2020 15:56:39 GMT

This compound is reactive and unstable and is therefore difficult to analyze by conventional mass spectrometry techniques. However, DART was easily able to detect propanethial S-oxide when a freshly cut chive bulb was placed in front of the mass spectrometer. The sample was analyzed at atmospheric pressure under ambient conditions and no sample preparation was required, other than cutting into the chive bulb. The compound was detected as [M+H]⁺ (C₃H₇SO⁺, m/z 91.0139).