PERFORMANCE
Sensitivity Boost
The liquid-nitrogen-cooled MARVEL HCN delivers more than twice the sensitivity of a room-temperature probe — a gain clearly visible in both the 1D comparison (Figure 1) and the 2D examples below. That improvement translates directly into more than a fourfold reduction in acquisition time for the same signal-to-noise.
Importantly, these are not idealized test scenarios. The spectra shown here were acquired on real samples under real-world experimental conditions, reflecting the performance users can expect in routine daily operation.
Figure 1. 1H spectrum of 4.5 mM vitamin B12 in D2O, acquired on room-temperature HCN and MARVEL HCN probes at 600 MHz under identical experimental conditions. S/N measurements repeated 10 times. MARVEL HCN / RT HCN S/N ratio of 2.25.
Figure 2. Two-scan 13C HSQC spectra of 4.5 mM vitamin B12 in D2O, acquired on room-temperature HCN and MARVEL HCN probes at 600 MHz. Experimental parameters were identical; data were scaled so that the RMS noise in both datasets is 1.0. Representative 1H slices through four resolved correlations (right) gave MARVEL HCN / RT HCN peak-height ratios of 2.01 to 2.23.
BIOMOLECULAR NMR
HCN Capability
The MARVEL HCN is a true triple-resonance probe, delivering the full range of 1H/13C/15N experiments required for biomolecular NMR. Figures 3 and 4 show a 15N SOFAST-HMQC and a 3D CBCA(CO)NH — examples of routine biomolecular experiments acquired on the MARVEL HCN.
Figure 3. 1H–15N SOFAST-HMQC spectrum of ubiquitin acquired on the MARVEL HCN probe at 600 MHz.
Figure 4. 3D CBCA(CO)NH spectrum of 0.35 mM ubiquitin acquired on the MARVEL HCN probe at 600 MHz.
BIOMOLECULAR NMR
Small-Molecule NMR
The MARVEL HCN performs equally well in routine small-molecule work and provides high-quality data.
Figure 5. Two-scan 13C HSQC spectrum of 4.5 mM vitamin B12 in D2O, acquired on the MARVEL HCN probe at 600 MHz.
Figure 6. 15N HSQC spectrum of 4 mM oxytocin in DMSO, acquired on the MARVEL HCN probe at 600 MHz in under 30 minutes.
Figure 7. 13C spectrum of 10 mg fluticasone propionate in DMSO, 256 scans, acquired on the MARVEL HCN probe at 600 MHz.
FLUORINE NMR
19F Capability
The inner high-frequency coil tunes to 19F, giving direct access to fluorine NMR on the same probe used for HCN work. This matters in pharmaceutical research, where an increasing proportion of drug candidates contain fluorine, and where 19F detection offers a clean, background-free window on the molecule.
Figure 8. 19F spectrum of 10 mg fluticasone propionate in DMSO, acquired on the MARVEL HCN probe at 600 MHz.
Figure 9. Two-scan 19F–13C HMBC spectrum of 10 mg fluticasone propionate in DMSO, acquired on the MARVEL HCN probe at 600 MHz. No 1JFC filter was applied, so both one-bond and long-range F–C correlations appear in a single experiment, mapping fluorine sites onto its surrounding carbon framework.