The MARVEL HCN probe is the latest addition to JEOL's SuperCOOL MARVEL family of liquid-nitrogen-cooled NMR probes. Building on the breakthrough performance of the original MARVEL and MARVEL HFX DUO probes, the MARVEL HCN extends JEOL's most advanced nitrogen-cooled probe technology to life-science NMR. This 5 mm triple-resonance probe covers 1H/19F, 13C and 15N, with cryogenic preamplifiers on both the 1H/19F and 13C channels providing enhanced sensitivity for proton- and carbon-detected experiments alike — more than twice the sensitivity of a room-temperature HCN probe, and over fourfold faster data acquisition.
Its innovative inner high-frequency (HF) coil can be tuned to either 1H or 19F, making the probe ideally suited for both HCN experiments and dedicated 19F NMR applications. This capability addresses the growing importance of fluorine analysis in pharmaceutical research, where an increasing proportion of modern drug candidates contain fluorine. The resulting flexibility supports a broad range of life-science applications, from routine small-molecule characterization and fragment screening to advanced biomolecular NMR studies — protein structure and backbone assignment, protein–ligand interactions, peptides, nucleic acids, and biologics characterization.
Figure 1. 1H spectrum of 4.5 mM vitamin B12 in D2O, acquired on room-temperature HCN and MARVEL HCN probes at 600 MHz under identical experimental conditions. S/N measurements repeated 10 times. MARVEL HCN / RT HCN S/N ratio of 2.25.
Figure 2. Two-scan 13C HSQC spectra of 4.5 mM vitamin B12 in D2O, acquired on room-temperature HCN and MARVEL HCN probes at 600 MHz. Experimental parameters were identical; data were scaled so that the RMS noise in both datasets is 1.0. Representative 1H slices through four resolved correlations (right) gave MARVEL HCN / RT HCN peak-height ratios of 2.01 to 2.23.
The MARVEL HCN is a true triple-resonance probe, delivering the full range of 1H/13C/15N experiments required for biomolecular NMR. Figures 3 and 4 show a 15N SOFAST-HMQC and a 3D CBCA(CO)NH — examples of routine biomolecular experiments acquired on the MARVEL HCN.
Figure 3. 1H–15N SOFAST-HMQC spectrum of ubiquitin acquired on the MARVEL HCN probe at 600 MHz.
Figure 4. 3D CBCA(CO)NH spectrum of 0.35 mM ubiquitin acquired on the MARVEL HCN probe at 600 MHz.
Figure 5. Two-scan 13C HSQC spectrum of 4.5 mM vitamin B12 in D2O, acquired on the MARVEL HCN probe at 600 MHz.
Figure 6. 15N HSQC spectrum of 4 mM oxytocin in DMSO, acquired on the MARVEL HCN probe at 600 MHz in under 30 minutes.
Figure 7. 13C spectrum of 10 mg fluticasone propionate in DMSO, 256 scans, acquired on the MARVEL HCN probe at 600 MHz.
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