SuperCOOL probes are available with two different cooling systems. The ideal choice for your lab will depend on the overall balance of cost, convenience, and performance.
The SuperCOOL circulating cooling system is a closed-cycle system that uses cooled helium gas to cool the critical probe and preamp components to less than 70 K. This results in a probe sensitivity that is around three times higher than the equivalent room temperature probe. The gas recirculating system ensures that running costs are relatively low and there is no need to replenish the coolant during routine operation. *1 Note: only 3rd generation SuperCOOL probe is currently available with circulating cooling system.
*1: Only during continuous operation. After stoppage, replenishment of coolant will be required.
JEOL cryogenic probes significantly reduce the 29Si background signals generated by probe components, enabling exceptionally clean measurements—even for glass materials. As shown in the spectra below, removing the sample tube results in a dramatic reduction of background signal, confirming that the majority of the 29Si interference originates from the tube itself rather than the probe.
SuperCOOL MARVEL is the latest generation of JEOL's liquid nitrogen cooled cryogenic probes and is the culmination of many years of our research and development in cryogenic cooling probe technology. It features a completely redesigned and optimized electrical circuit to achieve high sensitivity across a wide range of observation nuclides including 1H, 13C, 19F and 31P. The broadband design combines class-leading sensitivity across a wide range of nuclides with superior high-band performance, for an outstandingly powerful and versatile probe for both routine and advanced NMR applications, in a cost-effective and easy-to-use system.
The spectra below illustrate the excellent 13C sensitivity of SuperCOOL MARVEL. A series of 13C spectra of estradiol was collected at several different analyte concentrations. For quantities of material typically used to collect 13C spectra (~20 mg), SuperCOOL MARVEL can often obtain a high SNR 13C spectrum in under one minute. Even for sample amounts in the low mg range, SuperCOOL MARVEL can obtain a 13C spectrum in a few minutes, making it ideal for routine and high-throughput applications where sample amounts are limited.
500 MHz 1H NMR spectrum, 1 scan.
500 MHz 1H-13C DEPT-edited gradient HSQC, 32 scans per t1 increment, non-uniform sampling density 25%, reconstructed to 192 t1 increments.90 minute experiment time
500 MHz DEPT-edited HSQC spectrum. 4 scans per t1 increment, 128 t1 increments. Presaturation of the HOD signal was performed during the relaxation delay.33 minute experiment time
500 MHz 1H-15N sensitivity-enhanced HSQC spectrum. 2 scans per t1 increment, 42 t1 increments. 6 minute experiment time.
Below is a series of 13C spectra of fluticasone propionate collected with and without 1H and/or 19F decoupling. Used in conjunction with JEOL's ECZ or ECZ Luminous spectrometers, SuperCOOL MARVEL HFX Duo can perform triple-resonance HFX experiments, even with a standard two-channel system.
In the example shown, the use of simultaneous 1H and 19F decoupling simplifies the 13C spectrum by removing peak splittings and increases peak amplitudes, allowing the spectrum to be collected in significantly less time.
500 MHz 13C NMR spectra of fluticasone propionate collected with and without 1H and/or 19F decoupling.
Below is a standard 1H-13C multiplicity-edited HSQC spectrum of fluticasone propionate. The boxed region shows splittings in F1 due to one-bond scalar couplings between 13C and 19F
Standard 500 MHz 1H-13C edited HSQC spectrum of fluticasone propionate.
By adding a 19F broadband inversion pulse (BIP) at the middle of the t1 evolution period, these splittings are removed, simplifying the spectrum.
500 MHz 1H-13C edited HSQC spectrum of fluticasone propionate with an additional 19F BIP pulse at the mid-point of the t1 period.
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