Two-dimensional NMR experiments are incredibly useful for structure elucidation of complex molecules, especially when their one-dimensional spectra feature overlapping peaks. However, some experiments require significant amounts of time in order to yield data with adequate resolution or signal to noise for unambiguous interpretation. Any means of reducing the total acquisition time is useful. Non Uniform Sampling (NUS) is a method that can be used to speed up data collection and can be employed using our Delta™ software on JEOL Spectrometers.
Sampling of Indirect Dimensions
As an alternative, with Non Uniform Sampling (NUS), we can collect a smaller sample of the 1D spectra in the indirect dimension in order to speed up the experiment. In this example, we collect only a portion of the points (in Figure 2 below, 25%) and fill in the missing 1D increments with zeroes.
Because the total acquisition time in the 2nd dimension as well as the time steps between the samples are kept the same, the spectral width and resolution are identical to the uniformly sampled data.
Iterative Soft Thresholding
Iterative soft thresholding is a reconstruction technique for reconstructing the “missing” data points and then yields a conventional FID (Figure 3). See the "more information" section below for more information on the details of reconstruction.
The following spectra of lasalocid (acetone-d6) were all obtained on an ECZ400S equipped with a ROYAL probe. Directly below in figure 5 is a a typical 1H spectrum of lasalocid
Spectrum A in Figure 6 is a traditional, uniformly sampled C2HSQC with 128 Y points, a 200 ppm sweep width, 8 scans, and multiplicity editing. The total runtime of the experiment that produced the spectrum was approximately one hour. Note the congestion of the aliphatic region even in the 2D spectra. Spectrum B was acquired with a 25% sampling rate and all other parameters kept constant. Thus, the total acquisition time was cut down to 15 minutes. Spectra C and D maintained the 25% sampling, but increased the scans (C, to 32) or the Y points (512), in order to keep the total experiment time at one hour like spectrum A. Expansions of the aliphatic regions for these four spectra can be found in Figure 6. Note the increase in Y resolution (128 to 512 Y points) that is necessary to distinguish the overlapped peaks at 0.8 ppm as well as 1.3 ppm.
Figure 6. (Top) C2HSQC spectra of lasalocid at 400 MHz (Bottom) Expansion of the aliphatic region. Note the peaks that are resolved with 512 Y points (Spectrum D)
Below are expansions of the aliphatic regions from the above four spectra.
The ability to acquire multiplicity edited HSQCs (like the CRISIS-HSQCAD) with significantly reduced experiment time can be very valuable. For example, the spectrum of 50 mg of brucine (Figure 8) was acquired with 25% sampling, leading to a total experiment time of just under 5 minutes. In addition to giving 1H-13C connectivities, it also yields carbon multiplicity (methyls and methines phased opposite of methylenes, represented by blue and red peaks, respectively), rendering the acquisition of a slower, less sensitive 13C-detected DEPT unnecessary.
A Note of Caution
One should use caution regarding the sampling percentage chosen. For example, a system with multiple frequencies per Y slice (like an HMBC, or very congested HSQCs) can produce spectral artifacts during the reconstruction process when sampling rates are too low. In practice, this means ~25% for HSQCs and ~50% for HMBCs. While also possible, NUS is not currently recommended for homonuclear 2Ds like COSYs where there may be large number of correlations per Y slice, and NOESYs where the diagonal may be significantly more intense than the correlation peaks. As the field of NUS is one of ongoing research, the reader is encouraged to check the literature for new strategies in experimental design as well as data processing.
Employing Non Uniform Sampling allows for additional flexibility in total experiment time. One can increase scans to build up signal to noise or collect higher resolution data without sacrificing the other. As an alternative, one can obtain quicker “scout” 2Ds trading the potential for artifacts for quicker results. This can be useful to obtain a rough spectrum to see if running a much longer experiment is justified.
NUS is included in Delta. To download a free copy, please visit nmrsupport.jeol.com
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