Strengths of XtaLAB Synergy-ED
In the past, to perform structure analysis using 3D ED/MicroED, it was necessary to obtain diffraction patterns by using the camera and software provided with the electron microscope for photographing and process the data using another crystallographic software.
Most cameras are dedicated to obtaining electron microscopic images and are not suitable to obtain high quality electron diffraction that are required to analyze a single crystal structure.
To overcome this situation, HyPix-ED which is a world-reputed direct exposure photon counting detector in single crystal X-ray structure analysis field "HyPix" made applicable for electron measurement, and "CrysAlisPro" for ED which is software for 3D ED/MicroED based on data measurement and processing software "CrysAlisPro", were developed and installed in XtaLAB Synergy-ED.
Main Features of XtaLAB Synergy-ED
Seamless workflow from data measurement to crystal structure determination.
XtaLAB Synergy-ED has realized a seamless flow from measurement to analysis, which can visualize the 3D structure of atoms/molecules using electron beams.
A series of operations from sample selection, diffraction data acquisition, to structure analysis can be easily performed by CrysAlisPro for ED.
This is the outcome of combinations of RIGAKU's ultra-high sensitivity high speed detector"HyPix-ED" and dedicated single crystal structure analysis software "CrysAlisPro for ED" with JEOL’s technologies accumulated through transmission electron microscopes.
Structure determination is possible for small crystals of several hundreds nanometers or less, by utilizing the advantage of electron diffraction that can measure nano-sized crystals.
Single crystal electron diffraction analysis is not a substitute for single crystal X-ray structure analysis. It is an analytical technique suitable for determination of a small crystal structure which is difficult to measure using X-ray diffraction. This is possible because the interaction between an electron beam and atoms is thousands to tens of thousands stronger than with X-rays. The strong interaction is both an advantage and a disadvantage at the same time. One of the disadvantages is that there is an upper limit in the size (thickness) of crystals that can be measured. The size (thickness) of crystal measurable with 3D ED/MicroED is less than the limit measurable with X-rays. In terms of measurable crystal size, there is no overlapping between the two methods. In fact, they are complementary analysis methods.
Speedy and simpler measurement due to development of electron diffractometer specialized for electron diffraction.
3D ED/MicroED using a general-purpose transmission electron microscope requires knowledge and know-how of electron microscopy for setting up for transmission image acquisition and electron diffraction. The operation itself is often difficult for users who are not familiar with electron microscopy. For this reason, the XtaLAB Synergy-ED is specialized in functions as an electron diffractometer rather than an electron microscope, and integrates the CrysAlisPro for ED. This is optimized for electron diffraction, based on the CrysAlisPro, which has an established reputation for X-ray diffraction. The system allows the user to perform everything from crystal selection to data measurement, data processing and structural analysis in a single software package.Researchers who have used single crystal X-ray structure analysis can start analyzing the structure of a substance for which only nano-sized crystals are available immediately after installation.
Researchers who have used single crystal X-ray structure analysis, can immediately start using it. No need for the expertise of electron microscopy.
By integrating the flow from selection of measurement samples (nanocrystals) to data collection and analysis, electron diffraction can be easily used by non-specialists who lack the expertise in electron microscopy and crystallography that is conventionally required.
Structure analysis using XtaLAB Synergy-ED
Since its launch, XtaLAB Synergy-ED has determined one structure after another. More than 100 structures have already been determined by electron diffraction from nanocrystals. A wide range of samples have been analyzed, from MOFs and organic molecules to structures containing as many as 1,600 atoms per unit lattice.
Note: Refinement only based on kinetic theory.
Figure 1: a) number of atoms per unit lattice, b) R value obtained by refining based on kinetic theory for the structures that have been analyzed.
Since its launch, XtaLAB Synergy-ED has determined one structure after another. More than 100 structures have already been determined by electron diffraction from nanocrystals. A wide range of samples have been analyzed, from MOFs and organic molecules to structures containing as many as 1,600 atoms per unit lattice.
XtaLAB Synergy-ED supports very small crystals of a sample that has short and long lattices, various compositions
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