CRYO ARM™ 200S

Field Emission Cryo-electron Microscope
CRYO ARM™ 200S Field Emission Cryo-electron Microscope

One cryo-electron microscope for single-particle analysis, cryo-electron tomography and 3DED/MicroED

AI-driven automation and higher throughput make JEOL cryo-EM faster, more reproducible, and accessible to every operator. JEOL’s latest 200 kV cold field-emission cryo-electron microscope that offers single-particle analysis (SPA), cryo-electron tomography (cryo-ET), 3DED/MicroED and beyond, all in one highly automated instrument. The CRYO ARM™ 200S is engineered to deliver high-resolution structural data with the stability, throughput and ease of use that today’s structural-biology and drug-discovery laboratories require.

Features

CRYO ARM™ 200S × SmartScope

Single particle analysis data acquisition supported by AI

SmartScope¹ is an open-source software that supports data acquisition in single particle analysis. Using AI, it automatically detects areas suitable for imaging and can automate the entire process up to data acquisition. In addition, with an intuitive UI that organizes the operation flow in a simple manner and with necessary configurations kept to a minimum, even beginners can achieve stable data acquisition regardless of the level of experience.

1. Jonathan Bouvette et al., eLife, 11: e80047 (2022)

Workflow

Interface

CRYO ARM™ 200S × System Innovations

High throughput, easy operation

Smart sample transfer system CRYO SPECPORTER™-S

The advanced CRYO SPECPORTER™-S separates the storage for sample preservation from the transfer magazines used for sample exchange. This permits selective handling of only the samples required during analysis, eliminating the need to load and unload all samples in the instrument during exchange. This supports stable and efficient operation, even in multiuser environments.

Enhanced cartridge handling with higher transfer capacity

The number of cartridges that can be loaded into a magazine at one time has expanded from four to six, reducing the frequency and time required for sample exchange, boosting throughput while maintaining stable sample storage environment.

"Endless" multi-grid imaging capability

A flexible sample transfer system allows selective, individual exchange of only the required samples. In multi-grid imaging, samples analyzed can be exchanged as needed while new samples are preloaded in advance, allowing uninterrupted overnight operation and maximizing data acquisition efficiency.

Sample storage in a clean environment

The storage is maintained under vacuum and at liquid nitrogen temperature, enabling sample storage for approximately one month in a clean, ice-accumulation-free state. This allows flexible sample management in accordance with analysis schedules.

Beam designed for maximum information yield

CRYO ARM™ 200S features fringe-free illumination, which prevents interference fringes during imaging. By making efficient use of the beam, data can be acquired with high throughput while minimizing damage to the sample.

Improved flexibility of the electron beam

Seven optimized fringe-free beam conditions for each aperture size enable ideal illumination across a wide range of specimens.

Longer consumable life for reduced maintenance

Roots pumps are used for their extended maintenance intervals, reducing downtime and ensuring high system uptime.

Specifications

Main instrument

Electron gun Cold field-emission electron gun
Accelerating voltage 200 kV
Energy filter In-column Omega energy filter

Specimen Stage / Automated specimen exchange system

Specimen stage
Coolant Liquid nitrogen
Automated liquid-nitrogen filling system built-in
Specimen cooling temperature 100 K or less
Specimen movements
X、Y Motor drive (movements: ±1 mm)
Z Motor drive (movements: ±0.2 mm)
Tilt-X Motor drive (X-tilt:Not applicable[HR] / ± 70° [WG])
Specimen exchange system Air-lock
Automated cryo-transfer system built-in
Cooling temperature
(specimen exchange chamber)
105 K or less
Specimen exchange cartridge Up to 6 specimens can be exchanged at once.
Specimen parking stage Up to 12 specimens can be held.

Application Notes

Request CRYO ARM™ 200S Product Info / Virtual Demo

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