CRYO ARM™ 300S × SmartScope
Single particle analysis data acquisition supported by AI
SmartScope¹ is an open-source software that supports data acquisition in single particle analysis. Using AI, it automatically detects areas suitable for imaging and can automate the entire process up to data acquisition. In addition, with an intuitive UI that organizes the operation flow in a simple manner and with necessary configurations kept to a minimum, even beginners can achieve stable data acquisition regardless of the level of experience.
1. Jonathan Bouvette et al., eLife, 11: e80047 (2022)
Workflow
Interface
Result
Apoferritin Structure with SmartScope
CRYO ARM™ 300S × System Innovations
High throughput, easy operation
Smart sample transfer system CRYO SPECPORTER™-S
The advanced CRYO SPECPORTER™-S separates the storage for sample preservation from the transfer magazines used for sample exchange. This permits selective handling of only the samples required during analysis, eliminating the need to load and unload all samples in the instrument during exchange. This supports stable and efficient operation, even in multiuser environments.
Enhanced cartridge handling with higher transfer capacity
The number of cartridges that can be loaded into a magazine at one time has expanded from four to six, reducing the frequency and time required for sample exchange, boosting throughput while maintaining stable sample storage environment.
"Endless" multi-grid imaging capability
A flexible sample transfer system allows selective, individual exchange of only the required samples. In multi-grid imaging, samples analyzed can be exchanged as needed while new samples are preloaded in advance, allowing uninterrupted overnight operation and maximizing data acquisition efficiency.
Sample storage in a clean environment
The storage is maintained under vacuum and at liquid nitrogen temperature, enabling sample storage for approximately one month in a clean, ice-accumulation-free state. This allows flexible sample management in accordance with analysis schedules.
Beam designed for maximum information yield
CRYO ARM™ 300S features fringe-free illumination, which prevents interference fringes during imaging. By making efficient use of the beam, data can be acquired with high throughput while minimizing damage to the sample.
Improved flexibility of the electron beam
Improvements to the illumination system, including the condenser lens (CL) and CL aperture, refine the flexibility and stability of the electron beam. Enhanced lens demagnification ratio and aperture positioning accuracy improve beam positioning at the specimen plane. Eight aperture sizes and seven optimized fringe-free beam conditions for each aperture size enable ideal illumination across a wide range of specimens.
Longer consumable life for reduced maintenance
Roots pumps are used for their extended maintenance intervals, reducing downtime and ensuring high system uptime.
CRYO ARM™ 300S × CryoLameller
Cartridge-based cryo-CLEM workflow delivers high efficiency and high reproducibility
Integration with the CryoLameller (CRYO-FIB-SEM) enables a workflow that seamlessly connects sample preparation and analysis. From sample processing to data acquisition, the same cartridge holding the grid can be used throughout the workflow, allowing continuous operation while preserving positional information. With an integrated fluorescence microscope, intuitive and efficient area selection and positioning are achieved using correlative light and electron microscopy (CLEM).
CryoLameller – CRYO ARM™ Workflow
CLEM Application
CLEM imaging of fluorescently labeled mitochondria