Electron beam lithography uses a focused electron beam to pattern the surface of a material. As electron beams can be very tightly focused and small beam sizes achieved, electron beam lithography can be used to create very intricate structures for a wide variety of nanofabrication applications.

Professor Simon Watkins' lab at University of Pittsburgh, in collaboration with JEOL USA, has been developing novel ways to analyze biological structures in 3D, utilizing correlative FESEM and fluorescence microscopy. The paper “Correlative Fluorescence and Electron Microscopy in 3D – Scanning Electron Microscope Perspective” (Current Protocols in Cytrometry) describes how the ability to correlate fluorescence microscopy and electron microscopy data obtained on biological specimens bridges the resolution gap between the data obtained by these different imaging techniques.