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TEM vs. SEM Imaging: What's the Difference?

The key difference between SEM imaging and TEM is that SEM produces an image by detecting secondary or backscattered electrons, whereas TEM uses transmitted electrons to form an image.
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Understanding the Basics of 3D Electron Microscopy

A relatively novel frontier in improving the depth of metrological data that electron microscopy can provide is three-dimensional (3D) electron microscopy.