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How Cryo-EM Differs from TEM

How Cryo-EM Differs from TEM

Cryo-electron microscopy (cryo-em) and transmission electron microscopy (TEM) share many similarities, but their main difference is in sample prep.
Carbon Nanotubes imaged by TEM (L) and SEM (R)

Scanning Electron Microscopes Vs Transmission Electron Microscopes

Two of the most popular electron microscopy methods make use of scanning electron microscopes (SEM) and transmission electron microscopes (TEM).
SEM Image of Kidney Tissue.jpg

TEM vs. SEM Imaging: What's the Difference?

The key difference between SEM imaging and TEM is that SEM produces an image by detecting secondary or backscattered electrons, whereas TEM uses transmitted electrons to form an image.
3D SEM.jpg

Understanding the Basics of 3D Electron Microscopy

A relatively novel frontier in improving the depth of metrological data that electron microscopy can provide is three-dimensional (3D) electron microscopy.
Plum Island sand

Why is the Sand Purple at Plum Island Beach?

Typical New England beach sand differs in color from light and dark grey to medium tan based on its common mineralogy, but at Plum Island Beach there are swatches of purple sand that appear haphazardly as one walks along the shore.

Some Thoughts on Why You Want to Use Low kV Imaging

Some Thoughts on Why You Want to Use Low kV Imaging

What makes the difference between a good SEM image and a stellar one? Imaging samples at the appropriate conditions, and that often means at very low accelerating voltage (low kV). It's time to give it a try!

COVID-19 Virus using correlative FESEM and Fluorescence Microscopy

COVID-19 Virus Using correlative FESEM and Fluorescence Microscopy

Professor Simon Watkins' lab at University of Pittsburgh, in collaboration with JEOL USA, has been developing novel ways to analyze biological structures in 3D, utilizing correlative FESEM and fluorescence microscopy. The paper “Correlative Fluorescence and Electron Microscopy in 3D – Scanning Electron Microscope Perspective” (Current Protocols in Cytrometry) describes how the ability to correlate fluorescence microscopy and electron microscopy data obtained on biological specimens bridges the resolution gap between the data obtained by these different imaging techniques.

Paper Mask 1kV 350

Paper or Fabric?

During this unprecedented time when masks are being worn to help prevent the spread of COVID-19, we thought it would be interesting to take a closer look at masks. These were imaged on our IT500HR Scanning Electron Microscope at different magnifications.

JEM-1400

Delta College Adapts To New Routine With Online Microscopy Training And New Technology

Like any proud new owner, Cathy is anxious to get the new 1400, a 120kV TEM that is probably the most popular in its class for use in schools, hospitals, research labs, and materials, to work. She had a special plastic cover made for the JEM-1400 table, and it sits pristinely in the lab, yet idle, waiting for a new semester to begin whenever life returns to a new version of normal and schedules can resume.

Rhinoceros

Protecting Endangered Species – U.S. Fish and Wildlife Forensics Laboratory

The U.S. Fish and Wildlife Forensic Lab in Ashland, Oregon is helping to stop the export and import of rhino horn. The only wildlife forensic lab of its kind in the world, their work is identifying illegal exports and imports of products made from endangered wildlife as well as protected natural resources. JEOL is proud to share this story about how the AccuTOF-DART mass spectrometer is used to accurately identify the presence of rhino horn, as well as any suspicious items that may be made from endangered species, including pangolin and rare woods.

Thomas G. Huber: November 18, 1936 - December 2, 2019

Thomas G. Huber: November 18, 1936 - December 2, 2019

Thomas G. Huber: November 18, 1936 - December 2, 2019

Krish Krishnamurthy

CRAFT For NMR: Challenging Conventions To Achieve Faster, More Accurate Analysis

NMR data contains a trove of useful information for answering a wide variety of chemical and biological problems. However, with this broad utility comes complexity. CRAFT uses a Bayesian statistical approach to convert NMR time-domain data directly to the tabular domain. This bypasses the artifacts created by the Fourier transform when producing the spectrum and makes the automated extraction of these chemical shift and intensity data tables more reliable. With CRAFT, the spectrum (or frequency-domain data) is simply a human visualization tool for the data tables.

Naomi Miller holds sample of regolith and presented the findings of the CCMS-MIT research group at M&M 2019. Also in photo are her 8th grade teacher, Doug Shattuck (left) and JEOL collaborator Vern Robertson (right).

Middle School Students Evaluate How to Build Structures from Martian Soil

That’s the problem that 8th (now 9th) grade students at Concord-Carlisle Middle School, in collaboration with Massachusetts Institute of Technology and JEOL USA, set out to solve when they responded to a NASA challenge for the development of innovative technologies to support human colonization of Mars by 2050.

Guest Blog: Seeing Is Believing – How Benchtop SEMs Are Changing the Imaging Landscape

Guest Blog: Seeing Is Believing – How Benchtop SEMs Are Changing the Imaging Landscape

Traditional SEM instruments have provided us with unprecedented details of every surface you can think of, from whole insects to crystals and bacteria, but can be complex to use, requiring specialist knowledge, and also require a large dedicated space. The dawn and advancement of compact and user-friendly benchtop SEMs however is changing this picture.

The Nano Nemo on the Water - Armin VahidMohammadi

Science And Art Combine For Winning SEM Images

Entitled “Nano Nemo on the Water,” entry was selected as the winner of the Grand Prize SEM Image category in JEOL’s image contest for 2017.

Marine Science research yields winning SEM image

Marine Science Research Yields Winning SEM Image

Marine Science research yields winning SEM image

Image Contest Spotlight: Cian McKeown, University of Limerick

Image Contest Spotlight: Cian McKeown, University of Limerick

Cian has submitted 3 images that are all relevant to platinum nanostructures. We thought that the patterns that they form were interesting, so we asked the PhD candidate about his work with platinum.

Spider web prep

Imaging a Spider Web with the SEM

A special project for Concord Middle School students

Spider Silk 10,000X

Students Investigate Mechanical Properties of Spider Web

Research Scientists and Middle School Students Join Forces to Investigate Mechanical Properties of Spider Web

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