Analysis of the Natural Organic Compound SAAF Using the TOF-TOF Option
MSTips No. 183
Sperm activating and attracting factor (SAAF) is a naturally occurring substance produced by ascidians (sea squirts) to promote the fertilization process. Previously, the structure of this compound was characterized by high-energy collision induced dissociation (CID) using a traditional four sector tandem double focusing mass spectrometer.1 In this work, we analyzed the SAAF from a ciona intestinalis by using a JMS-S3000 SpiralTOF™ equipped with the optional TOF-TOF analyzer to generate a high energy CID product ion spectrum for this compound.