Analysis of oligonucleotides using JMS-S3000 "SpiralTOF™-plus 2.0"
MSTips No. 364
A nucleotide is a compound in which a phosphate group is bound to a nucleoside consisting of a base and a sugar and is a building block of deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). Nucleic acid drugs are attracting attention as therapeutic agents for diseases that have been difficult to treat, and as of October 2022, 16 nucleic acid drugs have been approved in Japan, U.S.A., or EU1). In recent years, synthesized oligonucleotides have been utilized as nucleic acid medicines. Molecular weight confirmation of synthesized oligonucleotides is important for the quality control of pharmaceuticals. In this report, we used the synthesized oligonucleotide (Table 1) as a sample and measured it with a MALDI-TOFMS.