Analytical Instrument Documents

This application note demonstrates a streamlined approach for determining both one-bond and long-range JCF values using 1D 13C NMR with selective 19F decoupling, enabled by the high 13C sensitivity and 1H&19F dual-channel decoupling capabilities of the SuperCOOL MARVEL HFX Duo probe.

JEOL's new SuperCOOL MARVEL probe offers exceptional sensitivity for both low-band (X) and high-band nuclei (1H/19F), enabling acquisition of INADEQUATE and ADEQUATE-style experiments on moderate sample amounts within reasonable timeframes. This note outlines practical guidelines for acquiring high-quality data using 25 mg of Estradiol (MW 272.38) using 500 MHz SuperCOOL MARVEL probe as an example.

In this Application Note we present an example of hot to efficiently obtain a set of full 13C & 1H assignments of Kanamycin-A disulfate (MW ~880 Da), using only 1 mg of the antibiotic and four basic NMR experiments and utilizing JEOL's new nitrogen-cooled SuperCool MARVEL probe.

Nitroxide radicals exhibit anisotropic g-values and hyperfine interactions depending on their molecular orientation relative to the magnetic field. However, in aqueous solutions, molecular motion is sufficiently fast on the ESR timescale to result in effective averaging of this orientational anisotropy. Consequently, motional narrowing occurs, as all molecules experience an equivalent magnetic field, leading to sharp ESR signals.

Sensitivity correction typically involves calibration using a standard sample with a known spin concentration, normalization with a Mn marker, normalization by sample mass, volume, or amount, and temperature correction based on the Boltzmann distribution.

This note highlights several key aspects of the SuperCOOL MARVEL probe’s performance that are central to modern NMR spectroscopy, including its sensitivity across multiple nuclei, its robust RF power‑handling capabilities, and its versatility in experiments involving 1H, 19F, and X‑nuclei.

ECZ Luminous™ Series Accessories Catalog

Scalar couplings arise between magnetically active nuclei that interact through chemical bonds. These couplings carry valuable information about molecular structure and conformation. Accurately measuring them, however, can be challenging. To overcome this, several NMR techniques have been developed, one of the most effective being the HECADE-HSQC (Heteronuclear Coupling Analysis via Double-Edited HSQC) experiment introduced by Koźmiński and Nanz.

The JNM-ECZL series is an FT NMR system equipped with state-of-the-art digital and high-frequency technologies. The new Multi Frequency Drive System enables multi-resonance measurements in a standard configuration, providing a wider range of solutions. Welcome to the world of ECZ Luminous™.

ECZ/ECZL Series daily inspection software that regularly diagnoses and calibrates the instrument, supporting stable daily operations and reducing the burden on instrument managers.