Analytical Instrument Documents

Application note NM260005
Product used: Nuclear Magnetic Resonance (NMR)

Classic INADEQUATE and ADEQUATE experiments are powerful tools for mapping the carbon backbone of organic molecules. However, their sensitivity is inherently low due to reliance on adjacent carbon atoms both being the rare ¹³C isotope - resulting in observable signals from only about 1 in 10,000 molecules.

JEOL's new SuperCOOL MARVEL probe offers exceptional sensitivity for both low-band (X) and high-band nuclei (¹H/¹⁹F), enabling acquisition of INADEQUATE and ADEQUATE-style experiments on moderate sample amounts within reasonable timeframes. This note outlines practical guidelines for acquiring high-quality data using 25 mg of Estradiol (MW 272.38) using 500 MHz SuperCOOL MARVEL probe as an example.