MALDI-ISD Measurements Using Both the SpiralTOF Mode and the LinearTOF Mode of JMS-S3000 “SpiralTOF™”
MSTips No. 229
Matrix-assisted laser desorption/ionization (MALDI) combined with in-source decay (ISD) is a useful tool for doing top-down sequencing of intact proteins. In this work, we measured and compared the ISD fragment ions generated for several proteins using the high resolution MALDI- Spiral mode and the high sensitivity MALDI-Linear mode available on the JEOL SpiralTOF MALDI-MS system.