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Structural Analysis of Triolein

Introduction:

The JMS-S3000 “SpiralTOF™” is a MALDI-TOF MS that uses an innovative spiral ion optical system to achieve the highest resolution currently available for a MALDI instrument. Additionally, this system can be equipped with a TOF-TOF option that can acquire high-energy collisioninduced dissociation (HE-CID) product ion spectra for monoisotopically selected precursor ions. The resulting HE-CID product ion spectra provide detailed structural information about compounds like triglycerides by means of charge-remote fragmentation (CRF).1 In this work, we report the structural analysis of triolein, a triglyceride that contains 3 oleic acid moieties (Fig. 1), by using HE-CID capabilities of the SpiralTOF-TOF.

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