Analytical Instrument Documents

MSTips No. 475

General

Gas chromatography-high resolution mass spectrometry (GC-HRMS) combined with nuclear magnetic resonance spectroscopy (NMR) is widely used for identifying chemical compositions and structures of organic synthetic compounds.

If a target sample is a high boiling point compound, which does not pass through GC, direct MS measurement is more suitable than GC-MS. The JMS-T2000GC, a GC-HRMS system, supports three different direct MS probes: 1) DIP (Direct Insert Probe) to heat a gas capillary containing a sample; 2) DEP (Direct Exposure Probe) with a filament; and 3) FDP (Field Desorption Probe) designed for field desorption, a soft ionization method. msFineAnalysis AI, designed to predict chemical compositions and formula, is effective in analyzing results acquired by direct MS as well as GC-MS, to determine desired NMR measurement methods and analyze the resulting NMR data.

JEOL’s ROYALPROBE^TM HFX, an accessory for the JNM-ECZ600R NMR system, is capable of tuning on the HF side (¹H,¹⁹F) for single and double resonance, allowing the operator to select one desired for the experiment. Combined with the LF side (¹³C, etc.), the probe supports simultaneous tuning for triple resonance, rendering itself as a powerful tool to determine chemical structures of organic synthetic compounds made of C, H, and F.

In this work, we examined a high boiling point compound in a JMS-T2000GC using a DEP, EI (DEI: Desorption Electron Ionization), FD (Field Desorption), and msFineAnalysis AI, and analyzed the resulting data in a JNM-ECZ600R with ROYALPROBE^TM HFX to determine its chemical composition and structure.