Analytical Instrument Documents

Edition August 2023

This is an application notebook that summaries a wide variety of applications of the JMS-S3000 SpiralTOF™ series MALDI-TOFMS that are useful as a shared instrument at universities.

Table of Contents

JMS-S3000 SpiralTOF™-plus 2.0 MALDI-TOFMS as a Shared Instrument　P1〜

Organic Chemistry　P3〜

• Accurate mass measurement of small molecules:
  Analysis of medicinal properties in a combination cold remedy by using JMS-S3000 "SpiralTOF™" (MSTips No. 241)

• Accurate mass measurement of a large molecule:
  Measurement of boroxine cage using JMS-S3000 "SpiralTOF™" (MSTips No. 369)

• Structural analysis of a small molecule:
  Structural analysis of a small molecule using JMS-S3000 "SpiralTOF™-plus 2.0"ーMS/MS measurement of a photodegradation product of reserpineー (MSTips No. 365)

Polymers / Oligomers / Surfactants　P9〜

• Molecular weight distributions of high molecular weight polymers:
  Analysis of high molecular weight polystyrene standards by using JMS-S3000 "SpiralTOF™" with Linear TOF option (MSTips No. 199)

• Analysis of anionic surfactants in negative ion detection mode:
  Structural analysis of anionic surfactants in MALDI negative ion mode using "SpiralTOF™- plus" (MSTips No. 333)

• Analysis of an insoluble polymer with solvent-free method:
  Analysis of low molecular weight polyethylene with solvent-free method using JMS-S3000 "SpiralTOF™" (MSTips No. 235)

Proteins / Peptides　P15〜

• Molecular weight measurement of proteins:
  High Sensitivity Analysis of Intact Proteins Using Linear TOF (MSTips No. 227)

• Identification of a protein with peptide mass fingerprinting (PMF) method:
  Analysis of Bovine Serum Albumin (MSTips No. 166)

• Identification of a protein with MS/MS ion search method:
  High-Energy CID Analysis of Bovine Serum Albumin (MSTips No. 174)

• Amino acid sequence analysis of a peptide:
  Structural Analysis of a High Molecular Weight Peptide (MSTips No. 175)

• Amino acid sequence analysis by using In-source Decay (ISD) method:
  MALDI-ISD Measurements Using Both the SpiralTOF™ Mode and the Linear Mode (MSTips No. 229)

Lipids　P25〜

• Structural analysis of phospholipids:
  Structural analysis of phospholipids in egg yolk using JMS-S3000 "SpiralTOF™" with TOFTOF option (MSTips No. 185)

Nucleic Acids　P27〜

• Accurate mass measurement of a synthetic nucleic acid:
  Analysis of oligonucleotides using JMS-S3000 "SpiralTOF™-plus 2.0" (MSTips No. 364)

Saccharides　P29〜

• Structural analysis of oligosaccharides:
  High-energy CID Mass Spectrometry of Oligosaccharides (MSTips No. 366)

Natural Products　P33〜

• Structural analysis of a natural product:
  Analysis of the Natural Organic Compound YTX by Using the TOF-TOF Option

Mass Spectrometry Imaging　P35〜

• High mass-resolution MS imaging of lipids in a mouse brain section:
  High mass-resolution MS imaging using JMS-S3000 "SpiralTOF™" and statistical data analysis (MSTips No. 370)