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Comparison of the JMS-S3000 SpiralTOF-TOF and a 4-Sector Tandem Double-Focusing Mass Spectrometer

Introduction

The JMS-3000 SpiralTOF™ has an optional TOF-TOF mode that features monoisotopic precursor ion selectivity, elimination of post source decay (PSD) ions, and high energy collision induced dissociation (CID). The JMS-700T MStation four-sector tandem double focusing mass spectrometer, although featuring a different analyzer and ionization techniques, has similar capabilities that have been previously used for a wide variety of applications including the structural analysis of complex biological molecules.

In this work, we compare the SpiralTOF-TOF with the MStation four sector tandem double focusing mass spectrometer using the same sample for MS/MS analysis.

Experimental

The sample analyzed by both systems was renin substrate tetradecapeptide (porcine), a peptide having an amino-acid sequence of DRVYIHPFHLLVYS. Table 1 shows the measurement conditions and sample sizes used for both systems.

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