It is difficult to handle the large size raw data, especially for a large number of mass spectrum points. It is difficult to use the detailed information from high mass resolution MALDI-Imaging by extracting mass images manually. Furthermore, peaks observed in the mass spectra cannot be identified by its origin, such as samples, matrix compounds or surface contaminations, before drawing the mass images. A lack of function to overview a large number of mass images. The JEOL msMicroImager software for high mass resolution MALDI-Imaging raw data was designed to resolve all of these issues.