Conversely, the JEOL JMS-S3000 “SpiralTOF™”, which has 5-10 times longer flight path than the traditional reflectron type TOF, is able to reduce the effect of this mass drift to achieve high mass resolution and high mass accuracy . The SpiralTOF is a MALDI-TOFMS that uses the unique JEOL spiral ion optical system which increases the ion flight path length to 17m. One consequence of this improvement in resolution is that there are more data points across each mass spectral peak, resulting in an overall increase in file size. This feature, along with the dimensions of the surface, the spatial resolution of each image data point, and the mass range measured, can all result in producing very large data files (10-20GB). Consequently, SpiralTOF imaging data generally requires file compression in order for the typical imaging software to reconstruct the MS images. However, a new software package called MALDIVision from PREMIER Biosoft  can easily handle these larger sized data files without the need for file compression. In this work, we used the SpiralTOF to collect the mass spectral data for a mouse brain section and then used MALDIVision to reconstruct the MS images using the uncompressed data files.