Analytical Instrument Documents

JEOL Resources

rss

Documentation in support of your JEOL product.

“Remainders of KM” plot for polymers using msRepeatFinder: compositional mapping over a broad mass range

Product: JMS-S3000

Introduction

Mass Spectrometry (MS) with soft ionization such as matrix-assisted laser desorption ionization (MALDI) allows the compositional analysis of polymers (repeating units, chain terminations) of low dispersity. Combining a size exclusion chromatography (SEC) fractionation with a high-resolution MALDI SpiralTOF™ MS analysis enables the evaluation of the composition of polydisperse polymeric samples over a broad mass range (high-resolution/high-accuracy mass measurements in the low mass range < 4 kDa, isotopic resolution in higher mass range < 30kDa). However, as the resolution gets higher, more peaks are detected in the mass spectrum for each fraction, making the interpretation of the mass spectral data the rate-limiting step for the whole analytical procedure. In this work, a “remainders of Kendrick mass” (RKM) analysis is used as a rapid post-acquisition data processing tool that uses visual maps from combined low/high-accuracy and low/high mass range data.

Experimental

A 1 mg mL-1 solution of poly(ɛ-caprolactone) (PCL, Polymer Source, P1302-CL) in CHCl3 was fractionated by SEC (HLC8220 GPC system, Tosoh, TSKgel multipore HXL-M columns, flow rate: 1 mL min-1, 0.5 mL per fraction). Mass spectra were recorded by using a JEOL JMS-S3000 SpiralTOF™ mass spectrometer (matrix: DCTB, no salt added). The plots were computed by using msRepeatFinder 3.0 (JEOL Ltd.).

Attached Files
Showing 0 Comment


Comments are closed.

Other Resources

Image Gallery
  • View a selection of NMR and MS spectra
  • Walkup NMR
  • See how the Delta NMR software allows users to just "walk up" and start NMR experiments
  • Mass Spec Reference Data
  • View our page of useful molecular references for Mass Spec
  • Tutorials (Mass Spec)
  • Documents on the basics of mass spectrometry
  • Delta NMR software Tutorials
  • Videos on how to use the Delta NMR software
  • No-D NMR
  • Description of No-D NMR and how it can be used to eliminate the need for deuterated solvents
  • Non Uniform Sampling (NUS)
  • Description of how NUS is used to greatly reduce the time needed for running NMR experiments
  • NMR Basics
  • Overview of the Basics of NMR Theory
  • NMR Magnet Destruction
  • See our presentation of the slicing open of a JEOL Delta-GSX 270 MHz NMR Magnet
  • Media

    Corona - Glow Discharge (DART Ion Source)

    January 28, 2022
    2637
      JEOL USA, Inc.
      11 Dearborn Road
      Peabody, MA 01960
      © Copyright 2022 by JEOL USA, Inc.
      Terms of Use
      |
      Privacy Policy
      |
      Cookie Preferences