Analytical Instrument Documents

JEOL Resources

rss

Documentation in support of your JEOL product.

“Remainders of KM” plot for polymers using msRepeatFinder: compositional mapping over a broad mass range

Product: JMS-S3000

Introduction

Mass Spectrometry (MS) with soft ionization such as matrix-assisted laser desorption ionization (MALDI) allows the compositional analysis of polymers (repeating units, chain terminations) of low dispersity. Combining a size exclusion chromatography (SEC) fractionation with a high-resolution MALDI SpiralTOF™ MS analysis enables the evaluation of the composition of polydisperse polymeric samples over a broad mass range (high-resolution/high-accuracy mass measurements in the low mass range < 4 kDa, isotopic resolution in higher mass range < 30kDa). However, as the resolution gets higher, more peaks are detected in the mass spectrum for each fraction, making the interpretation of the mass spectral data the rate-limiting step for the whole analytical procedure. In this work, a “remainders of Kendrick mass” (RKM) analysis is used as a rapid post-acquisition data processing tool that uses visual maps from combined low/high-accuracy and low/high mass range data.

Experimental

A 1 mg mL-1 solution of poly(ɛ-caprolactone) (PCL, Polymer Source, P1302-CL) in CHCl3 was fractionated by SEC (HLC8220 GPC system, Tosoh, TSKgel multipore HXL-M columns, flow rate: 1 mL min-1, 0.5 mL per fraction). Mass spectra were recorded by using a JEOL JMS-S3000 SpiralTOF™ mass spectrometer (matrix: DCTB, no salt added). The plots were computed by using msRepeatFinder 3.0 (JEOL Ltd.).

Attached Files
Showing 0 Comment


Comments are closed.

Other Resources

Walkup NMR
  • See how the Delta NMR software allows users to just "walk up" and start NMR experiments
  • Mass Spec Reference Data
  • View our page of useful molecular references for Mass Spec
  • Tutorials (Mass Spec)
  • Documents on the basics of mass spectrometry
  • Delta NMR software Tutorials
  • Videos on how to use the Delta NMR software
  • No-D NMR
  • Description of No-D NMR and how it can be used to eliminate the need for deuterated solvents
  • Non Uniform Sampling (NUS)
  • Description of how NUS is used to greatly reduce the time needed for running NMR experiments
  • NMR Basics
  • Overview of the Basics of NMR Theory
  • NMR Magnet Destruction
  • See our presentation of the slicing open of a JEOL Delta-GSX 270 MHz NMR Magnet
  • NMR Training
    Basic Operations and System Management for JEOL NMR Users
    Mass Spec Training
    Learn more about spectrometer operation and maintenance, data collection and processing, and advanced MS software operation.
    JEOLink NMR Newsletter
    We publish and send out this NMR newsletter to our customers. They can also be viewed here.
    Mass Media Newsletter
    We publish and send out this Mass Spec newsletter to our customers. They can also be viewed here.
    © Copyright 2024 by JEOL USA, Inc.
    Terms of Use
    |
    Privacy Policy
    |
    Cookie Preferences