Structural Analysis of a High Molecular Weight Peptide
Introduction:
The JMS-S3000 SpiralTOF™ is a MALDI-TOF MS that uses an innovative spiral ion optics system to achieve the highest resolution currently available for a MALDI instrument. Additionally, the JMS-S3000 is available with a TOF-TOF option that acquires high-energy collision-induced dissociation (CID) product-ion spectra for monoisotopically selected precursor ions. In this work, we analyzed a high molecular weight peptide by using the JMS-S3000 SpiralTOF with the TOF-TOF option.
Experimental:
Sample: ACTH18-39 (Arg-Pro-Val-Lys-Val-Tyr-Pro-Asn-Gly-Ala-Glu-Asp-Glu-Ser-Ala-Glu-Ala-Phe-Pro-Leu-Glu-Phe)
Matrix: α-Cyano-4-hydroxycinnamic acid (CHCA)