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Structural Analysis of Oxidized Triolein

Introduction:

In previous work, we showed that the JEOL SpiralTOFTOF system’s high-energy collision-induced dissociation (HE-CID) is useful for the structural analysis of triglycerides.1 The resulting HE-CID mass spectra provided detailed information about the fatty acid moieties such as the positions of double bonds, branching, hydroxylation, and oxidation by means of charge-remote fragmentation (CRF).2

In this work, we report the structural analysis of oxidized triglycerides by HE-CID using the SpiralTOFTOF.

Experimental:

Triolein, a triglyceride with 3 oleic acid moieties (Fig.1), was used in this experiment. The triolein sample was kept at 160°C for 60min so that thermal oxidation would occur. The resulting sample was then dissolved in tetrahydrofuran (THF) at a concentration of 10 mg/ mL. 2’,4’,6’-Trihydroxyacetophenone monohydrate (THAP) was used as the matrix and sodium trifluoroacetate (NaTFA) as the cationizing reagent. The THAP and NaTFA were dissolved in THF at a concentration of 10mg/mL and 1mg/mL, respectively. These two solutions and the sample solution were then mixed 1:1:1 by volume. Afterwards, 0.5 μL of this mixture solution was deposited and dried on the MALDI target plate.

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