Structural Analysis of Oxidized Triolein March 5, 2020 Application Note, MALDI SpiralTOF™-TOF, Mass Spectrometry (MS) 0 Introduction: In previous work, we showed that the JEOL SpiralTOFTOF system’s high-energy collision-induced dissociation (HE-CID) is useful for the structural analysis of triglycerides.1 The resulting HE-CID mass spectra provided detailed information about the fatty acid moieties such as the positions of double bonds, branching, hydroxylation, and oxidation by means of charge-remote fragmentation (CRF).2 In this work, we report the structural analysis of oxidized triglycerides by HE-CID using the SpiralTOFTOF. Experimental: Triolein, a triglyceride with 3 oleic acid moieties (Fig.1), was used in this experiment. The triolein sample was kept at 160°C for 60min so that thermal oxidation would occur. The resulting sample was then dissolved in tetrahydrofuran (THF) at a concentration of 10 mg/ mL. 2’,4’,6’-Trihydroxyacetophenone monohydrate (THAP) was used as the matrix and sodium trifluoroacetate (NaTFA) as the cationizing reagent. The THAP and NaTFA were dissolved in THF at a concentration of 10mg/mL and 1mg/mL, respectively. These two solutions and the sample solution were then mixed 1:1:1 by volume. Afterwards, 0.5 μL of this mixture solution was deposited and dried on the MALDI target plate. For full details: Attached files often contain the full content of the item you are viewing. Be sure and view any attachments. Apps Note Oxidized Triolein.pdf 725.73 KB Showing 0 Comment Comments are closed.