Introduction: The JMS-S3000 SpiralTOF™ is a MALDI-TOF MS that uses an innovative spiral ion optical system to achieve the highest resolution currently available for a MALDI instrument. Additionally, this system can be equipped with a TOF-TOF option that can acquire high-energy collisioninduced dissociation (HE-CID) product ion spectra for monoisotopically selected precursor ions. The resulting HE-CID product ion spectra provide detailed structural information about compounds like triglycerides by means of charge-remote fragmentation (CRF). 1 Tristearin is a triglyceride found in fats that consists of three stearic acid moieties (Fig. 1). In this work, we report the structural analysis of tristearin by using the HE-CID capabilities of the SpiralTOF-TOF.