Sudan Dyes Analysis by AccuTOFTM LC/MS System
Sudan dyes are red dyes that are used for coloring solvents, oils, waxes, petrol, and shoe and floor polishes. They are considered to be carcinogens and teratogens. Due to this fact, the US and the EU do not permit the use of these colors as food additives. However, in some countries, these dyes are still occasionally used in order to intensify the color of bell pepper and chili powders. Here, we describe a simple LC/TOF-MS method for sudan dyes I, II, III, and IV analysis.
All solvents used are of HPLC grade. Sudan dyes standards were prepared in methanol. The system consisted of an Agilent 1100 HPLC and JEOL AccuTOF™ time-of-flight mass spectrometer. The HPLC conditions and MS conditions are listed in Table 1 and Table 2, respectively.