Introduction: The JMS-S3000 “SpiralTOF™” is a MALDI-TOFMS that incorporates an innovative SpiralTOF ion optics system. This system is available with a TOF-TOF option that can acquire high-energy collision-induced dissociation (CID) product ion spectra for monoisotopically selected precursor ions. In this work, we analyzed Poly Methyl Methacrylate (PMMA) shown in Fig. 1 by using the JMS-S3000 SpiralTOF with the TOF-TOF option. The resulting high-energy CID data was then processed using the Polymerix™ (Sierra Analytics, Inc., http://massspec. com/) analysis software.