Electron Optic Documents

SerialEM, large files and robocopy

Tomographic and microED data sets created by SerialEM are single files with the data stored typically as 16-bit signed or unsigned integers. When these files have to be copied from the computer onto a USB drive, problems can arise.

SPA data collection with the Osaka framework

Cryo-EM has enjoyed an enormous ground swell in popularity ever since the advent of more stable and automated electron microscopes, suitable movie-type cameras, and improved acquisition software. Results obtained so far have been nothing short of spectacular as illustrated by several structures in EMDB and EMPIAR solved by cryo-EM to resolutions better than 1.5Å, such as EMD-31314, EMD-33707 and EMD-35984, the latter of which reaching true atomic resolution. This note describes the workflow used in Single Particle Analysis (SPA) cryo-EM workflows with the Osaka framework, i.e. a set of scripts that work with SerialEM.

STEM Damage Reduction using EDM Synchrony

Here we show an example of how Synchrony can reduce electron beam damage during atomic resolution STEM, by controlling with subatomic precision which regions are irradiated.

STEM Imaging for Electron-Beam Sensitive Sample Zeolite

Zeolites are sensitive to electron beam irradiation, making them very challenging samples for high-resolution imaging with a transmission electron microscope.

Sub-millisecond Time resolved TEM images of CeO2 with Relativity™ 250714

In this note, we report time-resolved TEM observations on the sub-millisecond order using the Relativity™ sub-framing system based on an electrostatic beam deflector manufactured by IDES.

TEM/STEM | LaB6

The 2100Plus is a multi-purpose 60-200kV TEM/STEM equipped with a LaB6. The 2100Plus enhances a laboratory's imaging capabilities. Learn more here.

TEMPO

TEMPO improves the amount of information obtained for a given electron dose, the information efficiency, of STEM experiments.

The development of a 200 kV monochromated field emission electron source

As seen in Ultramicroscopy, Volume 140, May 2014, Pages 37-43.

Time-resolved DPC imaging with EDM 250717

The Electrostatic Dose Modulator (EDM) makes stroboscopic measurements simple for TEM and STEM. In this application note, pulsed illumination boosts the time resolution of Differential Phase Contrast (DPC) imaging using the already-fast SAAF Quad segmented detector.

Transmission Electron Microscopy of Stainless Steel for Stents

TEM is an ideal technique for analyzing metals to gain an understanding of their structural and elemental properties on the sub-micrometer to atomic scale.

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