Electron Optic Documents

Tomographic and microED data sets created by SerialEM are single files with the data stored typically as 16-bit signed or unsigned integers. When these files have to be copied from the computer onto a USB drive, problems can arise.

Cryo-EM has enjoyed an enormous ground swell in popularity ever since the advent of more stable and automated electron microscopes, suitable movie-type cameras, and improved acquisition software. Results obtained so far have been nothing short of spectacular as illustrated by several structures in EMDB and EMPIAR solved by cryo-EM to resolutions better than 1.5Å, such as EMD-31314, EMD-33707 and EMD-35984, the latter of which reaching true atomic resolution. This note describes the workflow used in Single Particle Analysis (SPA) cryo-EM workflows with the Osaka framework, i.e. a set of scripts that work with SerialEM.

The 2100Plus is a multi-purpose 60-200kV TEM/STEM equipped with a LaB6. The 2100Plus enhances a laboratory's imaging capabilities. Learn more here.

As seen in Ultramicroscopy, Volume 140, May 2014, Pages 37-43.

TEM is an ideal technique for analyzing metals to gain an understanding of their structural and elemental properties on the sub-micrometer to atomic scale.


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