Electron Optic Documents

Using a multi-hole imaging scheme, researchers have been able to reach a hitherto unprecedented milestone of 20,000 images/day on both a CRYO ARM™ 300 II and a JEM-F200. Given that many structures on EMPIAR have required around 5000 images, essentially 4-5 projects can be accomplished on a daily basis, which opens up new opportunities for routine high resolution structure determination at unprecedented levels.

High resolution structure determination by electron cryo-microscopy (cryoEM) and Single Particle Analysis (SPA) has progressed to the point where structures can routinely be determined to be better than 2Å resolution using either a 200 or a 300 kV microscope. At 1.8Å resolution, details like amino acid isoforms can be distinguished. This application note highlights improved results that were obtained on apoferritin at 1.34Å resolution that hint at new features.

Auto tuning of aberration corrector

The field of single particle structure analysis (SPA) by cryo-electron microscopy reached new highs with the publication of a 1.19Å structure of apo-ferritin by Maki-Yonekura et al. obtained using a JEOL CRYO ARM™ 300 model 33001. This electron cryo-microscope was specifically designed for highly automated workflows capable of the unattended acquisition thousands of images of vitrified specimens. Workflow support of the JEOL CRYO ARM™ is available for SPA, batch tomography and microED.

Determining the near-atomic resolution structure of a biological macromolecule requires time on a high-end electron cryo-microscope. Depending on the local situation this could mean acquiring images of frozen-hydrated specimens on a JEOL CRYO ARM™ and/or another cryo microscope. To optimize inter-operability between different brands of cryo-microscopes, JEOL have investigated two related aspects: a) the reverse transfer, that is extracting frozen-hydrated specimens from one microscope to be transferred to another one, and b) the usability of a special cartridge designated as AG that are AutoGrid compatible.

Data set for observing ferromagnetic samples. The mode has no magnetic field around samples.

Luminary Micro is a Compact Specimen Photoexcitation System (CPXS) for JEOL TEMs. It is composed of a modulated laser, a compact optical delivery system, an inlet port, and a mirror. With this add-on, users can direct and focus the laser output onto the TEM sample in situ. Luminary Micro can induce a rich variety of reactions and dynamic processes in the specimen, thanks to its <40 μm FWHM focus size, adjustable peak power up to 3 W, and the modulated pulse widths ranging from a few microseconds to seconds. With Luminary Micro, users can study laser-induced phenomena in situ using fast cameras. Combined with IDES/JEOL EDM fast shutter and/or Relativity subframing systems, Luminary Micro allows users to perform time-resolved studies using pump-probe methods in the microsecond time scale. The extremely compact footprint of the system allows easy installation without affecting the TEM resolution. The user can heat specimens to thousands of degrees C while keeping the freedom to use the specimen holder of your choice.

Using Minimal Fringe Illumination and Coma-Free Image Shift an unprecedented throughput is possible on a JEOL CRYO ARM™. Given that a typical structure as published on EMPIAR requires 4-5000 images, the potential therefore exists of solving roughly 4-5 structures per day using a JEOL CRYO ARM™.

OBF System - Live Low Dose, Light Element Imaging

The IDES Relativity Electrostatic Subframing System multiplies the frame rate of cameras on JEOL TEMs. Microscopes equipped with Relativity achieve exceptional time resolution, data throughput, and advanced automation capabilities. Addition of Relativity allows current JEOL TEM users to forego expensive camera upgrades to their existing systems, instead relying on installation of an electrostatic optics assembly in a wide-angle camera port. These optics rapidly deflect the image data to different regions (subframes) of the camera in a programmable sequence. Each camera readout contains a tiled array of crisp, blur-free subframes. Raw data is automatically analyzed to give a stack of open format images that are loaded back into the camera control software for viewing or further analysis.

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