Analysis of Biological Fluids February 23, 2020 4. Forensics, AccuTOF™ DART®, Application Note, Mass Spectrometry (MS) 0 The AccuTOF-DART can detect a variety of substances in biological fluids such as urine, blood, and saliva with little or no sample preparation. These substances include drugs, amino acids, lipids, and metabolites. Urine samples were analyzed by dipping a melting point tube in urine and placing the sample in front of the DART ion source. Figure 1 shows positive-ion DART mass spectra of a urine sample from a subject taking ranitidine to reduce stomach acid production. The enlarged view in the inset shows the ranitidine metabolites desmethyl ranitidine and ranitidine N-oxide. For full details: Attached files often contain the full content of the item you are viewing. Be sure and view any attachments. Biological Fluids.pdf 185.59 KB Related Articles Analyzing a Specific Component using Group Analysis of msFineAnalysis Ver. 2 - MSTips 303 In 2018, we announced the msFineAnalysis software which was designed to automatically integrate two types of data acquired by EI and soft ionization. Recently, we developed msFineAnalysis Version 2, an enhanced version with additional features. msFineAnalysis Version 2 incorporates two new features: Chromatographic Deconvolution and Group Analysis. In this work, we use the group analysis capabilities of the software to evaluate the pyrolysis GC-MS results for a vinyl acetate resin. Integrated Analysis of an Acrylic Resin using msFineAnalysis v2 - MSTips 300 In 2018, msFineAnalysis Ver.1 software was released in which data acquired by EI, soft ionization, and accurate mass measurements were automatically integrated to generate a qualitative report for samples measured by these techniques with GC-MS. Recently, msFineAnalysis Ver.2 was introduced as an enhanced version with additional features. In this work, we will describe the changes in Ver.2, which now includes chromatographic deconvolution, and present applications using the new features. Integrated Analysis of Fatty Acid Methyl Esters using msFineAnalysis v2 - MSTips 301 Fatty acid methyl esters (FAMEs) are crucial for determining the fat content in food. Being environmentally friendly, they are also increasingly used as bio-diesel fuels. Many of the FAMEs are unsaturated with double bonds in the alkyl chains. As the number of double bonds increases (more unsaturation), the EI measurements tend to lack molecular ions. In this work, we measured a standard sample containing multiple FAMEs using EI and FI to detect their molecular ions. The resulting data was further examined by using msFineAnalysis to produce an integrated report for these compounds in which the library database search was combined with the molecular ion exact mass analysis to produce a qualitative identification of these compounds. Comprehensive Analysis + Unknown Component Analysis of Coffee Samples Using Headspace GC-MS Advances in mass spectrometry are enabling analysis of micro samples and unknown components that were not observable before. As the volume of information acquired from mass spectrometry increases, researchers are calling for simple techniques to analyze numerous components observed, and as a result, there is a rise in demand for comprehensive analytical techniques including multiple classification analysis. In this work, we will introduce a new technique of non-targeted analysis, which combines comprehensive analysis using high resolution GC-MS and unknown component analysis using soft ionization and EI. Comprehensive Analysis + Unknown Component Analysis of Vinyl Acetate Resins Using Pyrolysis GC-MS Advances in mass spectrometry are enabling analysis of micro samples and unknown components that were not observable before. As the volume of information acquired from mass spectrometry increases, researchers are calling for simple techniques to analyze numerous components observed, and as a result, there is a rise in demand for comprehensive analytical techniques including multiple classification analysis. In this work, we will introduce a new technique for non-target analysis, which combines comprehensive analysis using high resolution GC-TOFMS and unknown component analysis using soft ionization. Comprehensive 2D GC coupled with JEOL GC-HRTOFMS: GCxGC Applications Diesel Fuel Analysis by GCxGC/EI (Hydrocarbon Classification); Biomarker Analysis by GCxGC/PI (Target Analysis using 2D EICC); Type Analysis by GCxGC/FI (Hydrocarbon Type Analysis); Synthetic Polymer Analysis by Pryolysis GCxGC/EI and FI (Unknown Compounds Analysis in Nylon 66); Additives Analysis by Pyrolysis GCxGC/EI and FI (Targeted Additives Analysis in Nitrile Butadiene Rubber); Natural Polymer Analysis by Pyrolysis GCxGC/EI and PI (Powerful Separation of the Main Components in a Japanese Lacquer Film); Soluble Organic Fraction Analysis by GCxGC/EI (Analysis of PAHs in Exhaust Gas); Electronic Waste Analysis by GCxGC/EI and Negative CI (Halogenated Compounds Analysis); Aroma Oil (Fragrance) Analysis by GCxGC/EI and FI (Molecular ion detection for Alcohol compounds); Sebum Analysis by GCxGC/EI (Pharmaceutical application) Showing 0 Comment Comments are closed.