Analytical Instrument Documents

Application note NM260004
Product used: Nuclear Magnetic Resonance (NMR)

Fast and simple NMR Assignments of Antibiotic

Carbohydrate-based antibiotics are common and potent tools for fighting a large variety of infections. Repeating carbohydrtae fragments often result in significantly overlapping signal regions in ¹H NMR spectra of these compounds which complicates the NMR data analysis. Utilization of the added chemical shift dispersion of the ¹³C nuceli can simplify the assigment process at a cost of reduced sensitivity caused by low ¹³C natural abundance. A boost in sensitivity provided by JEOL's modern cryogenically cooled NMR probe technology dramatically reduces the time and effort required to assign NMR signals.

In this Application Note we present an example of hot to efficiently obtain a set of full ¹³C&¹H assignments of Kanamycin-A disulfate (MW ~880 Da), using only 1 mg of the antibiotic and four basic NMR experiments and utilizing JEOL's new nitrogen-cooled SuperCool MARVEL probe.