Compositional and Structural Analysis of Labeled N‑Linked Glycans Using JMS‑S3000 NewSpiralTOF™
Introduction
N‑linked glycans are post‑translational modifications in which N‑acetylglucosamine (GlcNAc) is attached to asparagine residues of proteins. They play important roles in protein structure and function and are involved in biological processes such as signal transduction and immune responses. Structural changes in N‑linked glycans are closely associated with various diseases, and they have attracted attention as biomarkers, particularly in cancer and congenital disorders of glycosylation. Therefore, accurate structural analysis of N‑linked glycans is important for both basic research and clinical applications. In this application note, labeled N‑linked glycans (Fig. 1) were analyzed using the JMS‑S3000, and results of accurate mass measurements in the SpiralTOF mode and structural analysis in the TOF‑TOF mode are presented.