Analytical Instrument Documents

JEOL Resources


Documentation in support of your JEOL product.

GC-MS Analysis of Cocoa Butter Triglycerides


Cocoa butter is the edible fat extracted from cocoa beans that is used in the manufacture of chocolate. Cocoa Butter Equivalents (CBE) are a substance derived from palm oil and shea butter that are used as a substitute for cocoa butter in chocolate. European regulations govern the labeling of chocolate as containing CBE less than 5%. US regulations require that Cocoa Butter Equivalents be specifically labeled with wording such as “chocolate flavored” coating. Analysis of the triglyceride content of chocolate is a quality control measure. Analysis of a standard sample of cocoa butter triglycerides is presented here as an example of the use of the JEOL Q1500 quadrupole GC-MS system for the detection of lipids separated by using a high-temperature GC column (Restek Rtx-65TG).


A certified cocoa butter reference standard (IRMM-801) was purchased from LGC and prepared as a solution in isooctane according to the directions supplied with the standard. A Restek Rtx-65TG high-temperature GC column was installed in the Agilent 7890B gas chromatograph with a 1 m deactivated fused silica guard column, and the column was directed into the JEOL MS-Q1500GC quadrupole mass spectrometer for analysis. The GC conditions (Table 1) were adapted from the European standard for the analysis of Cocoa Butter Equivalents ( One microliter of sample was introduced into the gas chromatograph as a split injection using a 10:1 split. The low split ratio causes a modest amount of overloading for the high-abundance components, but was used to make it easier to detect the low-level triglycerides in the total ion current chromatogram. Mass spectra were acquired in scan mode using the conditions shown in Table 2.

Attached Files
Showing 0 Comment

Comments are closed.

Other Resources

Walkup NMR
  • See how the Delta NMR software allows users to just "walk up" and start NMR experiments
  • Mass Spec Reference Data
  • View our page of useful molecular references for Mass Spec
  • Tutorials (Mass Spec)
  • Documents on the basics of mass spectrometry
  • Delta NMR software Tutorials
  • Videos on how to use the Delta NMR software
  • No-D NMR
  • Description of No-D NMR and how it can be used to eliminate the need for deuterated solvents
  • Non Uniform Sampling (NUS)
  • Description of how NUS is used to greatly reduce the time needed for running NMR experiments
  • NMR Basics
  • Overview of the Basics of NMR Theory
  • NMR Magnet Destruction
  • See our presentation of the slicing open of a JEOL Delta-GSX 270 MHz NMR Magnet
  • NMR Training
    Basic Operations and System Management for JEOL NMR Users
    Mass Spec Training
    Learn more about spectrometer operation and maintenance, data collection and processing, and advanced MS software operation.
    JEOLink NMR Newsletter
    We publish and send out this NMR newsletter to our customers. They can also be viewed here.
    Mass Media Newsletter
    We publish and send out this Mass Spec newsletter to our customers. They can also be viewed here.
    © Copyright 2024 by JEOL USA, Inc.
    Terms of Use
    Privacy Policy
    Cookie Preferences