In this work, we report the identification of impurities and degradation products of Ziracin, a member of the Everninomicin class of antibiotics. Everninomicins belong to an important group of oligosaccharides, isolated from the fermentation broth, Micromonospora Carbonaceae , and are found to be highly active against Gram-positive bacteria including Methicillin resistant Staphylococci and Vancomycin resistant Enterococci . With the emergence of drug resistant strains of bacteria, a major effort is being directed towards the reevaluation of the efficacy of existing oligosaccarides and identification of new potential oligosaccaride antibiotics. Currently, the Everninomicin antibiotic Ziracin (SCH27889, Schering-Plough Corporation) is undergoing extensive trials to determine its clinical efficacy. Preliminary results suggest that Ziracin is safe and effective and if it proves successful in the large scale clinical trials, it could become a very important drug for treatment of human infections.