Analytical Instrument Documents

JEOL Resources

rss

Documentation in support of your JEOL product.

High Sensitivity Peptide Measurement with the New Matrix α-Cyano-4-Chlorocinnamic Acid

Introduction:

Matrix assisted laser desorption ionization (MALDI) is a powerful and useful ionization technique that is commonly used for the analysis of biomolecules such as peptides and proteins. Typically, α-Cyano-4-hydroxycinnamic acid (CHCA) is the matrix used for MALDI peptide measurement. Recently, a new matrix “α-Cyano-4-chlorocinnamic acid (CClCA)” was investigated for peptide analysis [1]. In this study, we demonstrate the measurement of a BSA digest to evaluate the improvement in peptide sensitivity with CClCA in comparison with CHCA by using the JMSS3000 SpiralTOF MS system.

Experimental:

Sample information and preparation conditions are shown in Table 1. The BSA digest was obtained from Michrom. The CHCA matrix and peptide standard were obtained from Sigma-Aldrich, and CClCA matrix was obtained from Cayman.

Attached Files
Showing 0 Comment


Comments are closed.

Other Resources

Image Gallery
  • View a selection of NMR and MS spectra
  • Walkup NMR
  • See how the Delta NMR software allows users to just "walk up" and start NMR experiments
  • Mass Spec Reference Data
  • View our page of useful molecular references for Mass Spec
  • Tutorials (Mass Spec)
  • Documents on the basics of mass spectrometry
  • Delta NMR software Tutorials
  • Videos on how to use the Delta NMR software
  • No-D NMR
  • Description of No-D NMR and how it can be used to eliminate the need for deuterated solvents
  • Non Uniform Sampling (NUS)
  • Description of how NUS is used to greatly reduce the time needed for running NMR experiments
  • NMR Basics
  • Overview of the Basics of NMR Theory
  • NMR Magnet Destruction
  • See our presentation of the slicing open of a JEOL Delta-GSX 270 MHz NMR Magnet
  • Media

    Corona - Glow Discharge (DART Ion Source)

    January 28, 2022
    2637
      JEOL USA, Inc.
      11 Dearborn Road
      Peabody, MA 01960
      © Copyright 2022 by JEOL USA, Inc.
      Terms of Use
      |
      Privacy Policy
      |
      Cookie Preferences