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High Sensitivity Peptide Measurement with the New Matrix α-Cyano-4-Chlorocinnamic Acid


Matrix assisted laser desorption ionization (MALDI) is a powerful and useful ionization technique that is commonly used for the analysis of biomolecules such as peptides and proteins. Typically, α-Cyano-4-hydroxycinnamic acid (CHCA) is the matrix used for MALDI peptide measurement. Recently, a new matrix “α-Cyano-4-chlorocinnamic acid (CClCA)” was investigated for peptide analysis [1]. In this study, we demonstrate the measurement of a BSA digest to evaluate the improvement in peptide sensitivity with CClCA in comparison with CHCA by using the JMSS3000 SpiralTOF MS system.


Sample information and preparation conditions are shown in Table 1. The BSA digest was obtained from Michrom. The CHCA matrix and peptide standard were obtained from Sigma-Aldrich, and CClCA matrix was obtained from Cayman.

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