Qualitative and Quantitative Analysis of Organic Acids in Urine by Gas Chromatography-High Resolution Mass Spectrometry with Hard Ionization and Soft Ionization
Introduction
The test of urine organic acids is an essential component of the study of inherited metabolic diseases, such as inborn error of metabolism. Urine contains a variety of organic acids that forms through diverse sources, including normal and abnormal metabolism, drugs and drug metabolism, or even xenobiotics and dietary supplements. The gas chromatography - mass spectrometry (GC-MS), especially GC - single quadrupole MS(GC-QMS) is generally chosen for the analysis of urine organic acids. However, since GC-QMS lacks the ability to the accurate identification of organic acids in urine due to its complicated mixture especially when creating a new method, GC-high resolution MS (GC-HRMS) is a more qualified system instead.
JMS-T2000GC, a GC-HRMS system that incorporates a combination ion source of electron ionization (EI) and field ionization (FI), is capable of switching between the EI and FI modes without breaking the vacuum. The analysis of data acquired by the 2 ionization methods through msFineAnalysis AI software leads to the estimation of chemical structures as well as compositions, which is practical for the determination of compounds in organic acids.
In this work, the qualitative analysis of the chemical composition and structure of organic compounds in urine and the quantitative analysis of target organic acid using JMS-T2000GC with msFineAnalysis AI was executed.