Rapid Detection of Fungicide in Orange Peel March 4, 2020 3. Food, Flavors, Fragrances, AccuTOF™ DART®, Application Note, Mass Spectrometry (MS) 0 Thiabendazole is an anthelmintic and a highly persistent systematic benzimidazole fungicide that is widely used for controlling spoilage in citrus fruit. It is considered a General Use Pesticide (GUP) in EPA Toxicity Class III – Slight Toxicity. A small piece of orange peel (a few square millimeters in size) from a Florida orange was placed in the DART sampling region. Compounds present in the peel were detected within seconds. Among these were the familiar orange-oil flavor components such as limonene and sinensal as well as polymethoxylated flavones that are attributed with antioxidant and cholesterol-reducing properties. For full details: Attached files often contain the full content of the item you are viewing. Be sure and view any attachments. Orange Peel.pdf 57.49 KB Related Articles Rapid detection of fentanyl, fentanyl analogues, and opioids for on-site or laboratory based drug seizure screening using thermal desorption DART-MS and ion mobility spectrometry Fentanyl and fentanyl analogues represent a current and emerging threat in the United States as pure illicit narcotics and in mixtures with heroin. Because of their extreme potency, methods to safely and rapidly detect these compounds are of high interest. This work investigates the use of thermal desorption direct analysis in real time mass spectrometry (TD-DART-MS) and ion mobility spectrometry (IMS) as tools for the rapid and sensitive (nanogram to picograms) detection of fentanyl, 16 fentanyl analogues, and five additional opioids. Competitive ionization studies highlight that detection of these compounds in the presence of heroin is readily achievable, down to 0.1% fentanyl by mass with TD-DART-MS. With IMS, detection of nanogram levels of fentanyl in a binary fentanyl and heroin mixture is possible but can be complicated by decreased resolution in certain commercial instrument models. Modifications to the alarm windows can be used to ensure detection of fentanyl in binary mixtures. Additionally, three complex background matrices (fingerprint residue, dirt, and plasticizers) are shown to have a minimal effect of the detection of these compounds. Wipe sampling of the exterior of bags of questioned powders is shown to be a safe alternative method for field screening and identification, removing the need to handle potentially lethal amounts of material. Rapid Detection of Melamine in Dry Milk Using AccuTOF-DART Recent events have led to the recall of both pet food and dairy food products from international consumer markets. In both cases, melamine was added to these products to show a higher chemical signature for proteins, which in turn would increase the reported quality of the food. Unfortunately, the effect of this melamine addition caused the death of both pets and babies that consumed these tainted products. As a result, there is growing government and consumer concern towards the presence of melamine in food products. Because of this concern, there is a need for a rapid and accurate test to quickly determine the presence of melamine in these food products. Previously, the JEOL AccuTOF-DART was shown to be an effective technique for determining the presence of melamine in pet food. In this work, we extend the application of AccuTOF-DART to show that melamine can be rapidly detected when it is present in dry nonfat milk. Rapid Detection and Exact Mass Measurements of Trace Components in an Herbicide Analytical chemists are often asked to identify trace components in manufactured compounds such as drugs, consumer products, and agricultural chemicals. A common approach to the identification of minor components is to use gas or liquid chromatography coupled with high-resolution mass spectrometry. Although this approach is effective, it may be time consuming and difficult to set up. The AccuTOF with Direct Analysis in Real Time (DART™) provides a rapid solution. The high dynamic range of both source and detector permit the determination of minor components in the presence of a major component. The AccuTOF always provides high resolution data with exact mass measurements and accurate isotope ratios that can provide elemental composition assignments for unknown compounds. Instantaneous Detection of the “Date-Rape” Drug -- GHB Gamma hydroxybutyrate (GHB) is a fast-acting central nervous system depressant. Prior to its ban by the FDA in 1990, GHB was sold in bodybuilding formulas. It has been abused as a euphoriant. Because it is colorless and odorless, it can be added to alcoholic drinks of unsuspecting victims. An overdose can result in serious consequences, including respiratory depression and coma. GHB was classified as a Schedule I Controlled Substance in March, 2000. Detection of GHB is problematic. GC/MS and LC/ MS methods are time consuming. A rapid colorimetric assay for GHB has been developed, but this assay suffers from some limitations. For example, ethanol produces the same colorimetric response as GHB. The AccuTOF™ mass spectrometer equipped with Direct Analysis in Real Time (DART™) can rapidly detect GHB anion (C4H7O3 -, m/z 103.0395) on surfaces, in urine, and in ethanol. No solvent extraction, wipes, or chromatography are required. Examples are shown in the figures below. Rapid Characterization of Bacteria Using ClairScope™ and SpiralTOF™ In many fields such as clinical diagnosis and food inspection, there is a demand for rapid, reliable and simple-to-use methods for characterizing bacteria. This paper explores the use of two new and innovative instruments called ClairScope™ and SpiralTOF™ for this rapid characterization. The JASM-6200 ClairScope™ integrates an optical microscope (OM) with a scanning electron microscope (SEM) where it is possible to observe samples in solution, in an open system, by the SEM at atmospheric pressure. This type of sample would typically require extensive sample pretreatment that would take a day or more with conventional SEM techniques. With the recently developed ClairScope™, fine morphological observation can be performed directly in solution with simple sample pretreatment of one hour or less. The JMS-S3000 SpiralTOF™, is a matrix-assisted laser desorption/ionization mass spectrometer (MALDI-MS) with a spiral ion trajectory. With sample pretreatment as fast as a few minutes, the SpiralTOF™ can characterize ribosomal proteins and phospholipids with high accuracy. Ribosomal proteins are biomarkers for phylogenetic classification, and phospholipids are used for chemotaxonomic analysis. The combination of ClairScope™ and SpiralTOF™ are found to be powerful instruments for the characterization of bacteria. Detection of Explosives in Muddy Water The AccuTOF time-of-flight mass spectrometer equipped with Direct Analysis in Real Time (DART™) has been used to detect a wide variety of explosives in or on a variety of materials ranging from solutions to samples deposited on surfaces ranging from ABS plastic to metal, clothing and cardboard. Detection is rapid, specific, and sensitive. Showing 0 Comment Comments are closed.