Analytical Instrument Documents

Tandem mass spectrometry is a powerful tool for polymer characterization. It can obtain information about polymer end groups, repeating structures (linear, cyclic, or branched), and copolymerization. High-energy collision–induced dissociation (HE-CID) is a fragmentation method that is available only in tandem time-of-flight mass spectrometry (TOF–TOF). The informative fragmentation channels, which are difficult to observe with commonly used low-energy CID, are often observable in HE-CID spectra. In MSTips 270, we proposed a method to visualize this abundant structural information and enable intuitive analysis using the “Remainders of KM” (RKM) plot method. In this report, we applied the method to analyze polyethylene oxide (PEO) with different end groups.

The JMS-S3000 “SpiralTOF™” is a MALDI-TOFMS that incorporates an innovative SpiralTOF ion optics system. This system is available with a TOF-TOF option that can acquire high-energy collision-induced dissociation (CID) product ion spectra for monoisotopically selected precursor ions. In this work, we analyzed Poly Methyl Methacrylate (PMMA) shown in Fig. 1 by using the JMS-S3000 SpiralTOF with the TOF-TOF option. The resulting high-energy CID data was then processed using the Polymerix™ (Sierra Analytics, Inc., http://massspec. com/) analysis software.

Various kinds of additives are used in a polymer, such as an antioxidant, a light stabilizer, and an ultraviolet absorber. Because a polymer’s properties depend on the additives in the raw material, it is important to understand these additives. Matrix-assistance laser desorption/ ionization time-of-flight mass spectrometer (MALDI-TOFMS) JMS-S3000 SpiralTOF™ is widely used in polymer analysis. High-energy collision–induced dissociation (HE-CID) measurement with the TOF-TOF option is also useful in analyzing the structures of additives. SpiralTOF™ can achieve a high precursor ion selection with a revolution of 17 m. Fragmentation derived from HE-CID can be observed only due to the ions from post-source decay (PSD), these fragment ions can be eliminated by the four electrostatic sectors that constitute the spiral trajectory. In this Applications Note, we report on polymer structural analysis by compare fragmentation patterns in the product ion spectra of IRGANOX 1076 M+・ and [M+Na]+.

The JMS-S3000 SpiralTOF™ is a MALDI-TOF MS that uses an innovative spiral ion optical system to achieve the highest resolution currently available for a MALDI instrument. Additionally, this system can be equipped with a TOF-TOF option that can acquire high-energy collisioninduced dissociation (HE-CID) product ion spectra for monoisotopically selected precursor ions. The resulting HE-CID product ion spectra provide detailed structural information about compounds like triglycerides by means of charge-remote fragmentation (CRF).1 Tristearin is a triglyceride found in fats that consists of three stearic acid moieties (Fig. 1). In this work, we report the structural analysis of tristearin by using the HE-CID capabilities of the SpiralTOF-TOF.

The JMS-S3000 “SpiralTOF™” is a MALDI-TOF MS that uses an innovative spiral ion optical system to achieve the highest resolution currently available for a MALDI instrument. Additionally, this system can be equipped with a TOF-TOF option that can acquire high-energy collisioninduced dissociation (HE-CID) product ion spectra for monoisotopically selected precursor ions. The resulting HE-CID product ion spectra provide detailed structural information about compounds like triglycerides by means of charge-remote fragmentation (CRF). In this work, we report the structural analysis of triolein, a triglyceride that contains 3 oleic acid moieties (Fig. 1), by using HE-CID capabilities of the SpiralTOF-TOF.

In previous work, we showed that the JEOL SpiralTOFTOF system’s high-energy collision-induced dissociation (HE-CID) is useful for the structural analysis of triglycerides. The resulting HE-CID mass spectra provided detailed information about the fatty acid moieties such as the positions of double bonds, branching, hydroxylation, and oxidation by means of charge-remote fragmentation (CRF). In this work, we report the structural analysis of oxidized triglycerides by HE-CID using the SpiralTOFTOF.

The JMS-S3000 SpiralTOF™, which has a JEOL-patented spiral orbital ion optical system, has a long-flight distance of 17 m and can achieve high-mass resolution. In addition, the electrostatic sectors that constitute the ion optical system can exclude PSD ions, making it possible to acquire a mass spectrum that is less affected by background ions. These features enable accurate mass measurement even in LMW regions[1].

The JMS-S3000 SpiralTOF™ is a MALDI-TOF MS that uses an innovative spiral ion optics system to achieve the highest resolution currently available for a MALDI instrument. Additionally, the JMS-S3000 is available with a TOF-TOF option that acquires high-energy collision-induced dissociation (CID) product-ion spectra for monoisotopically selected precursor ions. In this work, we analyzed a high molecular weight peptide by using the JMS-S3000 SpiralTOF with the TOF-TOF option.

Includes 32 application notes in the following categories: Introduction and Fundamentals, Synthetic Polymers, Small Molecules, Lipids, Peptides and Proteins, Other biomolecules (natural products, oligosaccharides), and JMS-S3000 SpiralTOF bibliography

Tandem mass spectrometry of polymer ions provides valuable information about the nature of individual end-groups, chain architecture (linear / cyclic / branched) and copolymeric microstructure. Using matrix-assisted laser desorption ionization (MALDI) with high energy collision-induced dissociation (HE-CID) tandem time-of-flight analysis (TOF/TOF) can be advantageous for MS/MS polymer analysis. However, the interpretation of the resulting mass spectrum may not be obvious when a number of ion series with low intensity signals are present. In this work, a “remainders of Kendrick mass” (RKM) analysis is used as a rapid post-acquisition data processing tool for TOF/TOF mass spectra in order to visualize and filter the ion series instantly via intuitive point alignments.

rss

Other Resources

Image Gallery
  • View a selection of NMR and MS spectra
  • Walkup NMR
  • See how the Delta NMR software allows users to just "walk up" and start NMR experiments
  • Mass Spec Reference Data
  • View our page of useful molecular references for Mass Spec
  • Tutorials (Mass Spec)
  • Documents on the basics of mass spectrometry
  • Delta NMR software Tutorials
  • Videos on how to use the Delta NMR software
  • No-D NMR
  • Description of No-D NMR and how it can be used to eliminate the need for deuterated solvents
  • Non Uniform Sampling (NUS)
  • Description of how NUS is used to greatly reduce the time needed for running NMR experiments
  • NMR Basics
  • Overview of the Basics of NMR Theory
  • NMR Magnet Destruction
  • See our presentation of the slicing open of a JEOL Delta-GSX 270 MHz NMR Magnet
  • Media

    Corona - Glow Discharge (DART Ion Source)

    January 28, 2022
    2714
    JEOL Ltd. global website
      JEOL USA, Inc.
      11 Dearborn Road
      Peabody, MA 01960
      © Copyright 2023 by JEOL USA, Inc.
      Terms of Use
      |
      Privacy Policy
      |
      Cookie Preferences