Analytical Instrument Documents

MALDI-ISD Measurements Using Both the SpiralTOF Mode and the LinearTOF Mode of JMS-S3000 “SpiralTOF™”
MALDI-ISD Measurements Using Both the SpiralTOF Mode and the LinearTOF Mode of JMS-S3000 “SpiralTOF™”

Matrix-assisted laser desorption/ionization (MALDI) combined with in-source decay (ISD) is a useful tool for doing top-down sequencing of intact proteins. In this work, we measured and compared the ISD fragment ions generated for several proteins using the high resolution MALDI- Spiral mode and the high sensitivity MALDI-Linear mode available on the JEOL SpiralTOF MALDI-MS system.

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High Sensitivity Analysis of intact Proteins Using Linear TOF
High Sensitivity Analysis of intact Proteins Using Linear TOF

In this work, we demonstrate the measurement of intact proteins by using the Linear TOF option for the JEOL SpiralTOF system.

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Rapid Confirmation of Synthetic Compounds Using DART™ –Direct Mass Spectrometric Analysis from NMR Sample Tubes
Rapid Confirmation of Synthetic Compounds Using DART™ –Direct Mass Spectrometric Analysis from NMR Sample Tubes

We report an application related to synthetic compound confirmation that combines nuclear magnetic resonance (NMR), which is indispensable for structural analysis of organic compounds, and mass spectrometry by DART.

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Analysis of cyanoacrylate adhesive using the JMS-S3000 “SpiralTOF™” — Application of Kendrick Mass Defect plot analysis
Analysis of cyanoacrylate adhesive using the JMS-S3000 “SpiralTOF™” — Application of Kendrick Mass Defect plot analysis

Recently, Kendrick mass defect (KMD) plot analysis has been proposed as an effective data processing technique for SpiralTOF™ which allows for the structural characterization of complicated compounds1, 2. Here, a commercial acrylic adhesive containing cyanoacrylate was characterized using a SpiralTOF™ combined with KMD plot analysis.

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Analysis of high molecular weight polystyrene standards by using JMS-S3000
Analysis of high molecular weight polystyrene standards by using JMS-S3000 "SpiralTOF™" with Linear TOF option

High molecular weight polystyrene standards are analyzed by using JMS-S3000 SpiralTOF™ with Linear TOF option.

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Structural Analysis of Oxidized Triolein
Structural Analysis of Oxidized Triolein

In this work, we report the structural analysis of oxidized triglycerides by HE-CID using the SpiralTOF-TOF.

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Analysis of Phosphopeptide using TOF-TOF
Analysis of Phosphopeptide using TOF-TOF

In this work, we measure a monophosphopeptide (FQ pS EEQQQTEDELQDK) that was obtained from the tryptic digestion of β-casein (Bovine) using the TOF-TOF option that is available for the JEOL SpiralTOF™ system.

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Analysis of the Natural Organic Compound SAAF Using the TOF-TOF Option
Analysis of the Natural Organic Compound SAAF Using the TOF-TOF Option

In this work, we analyzed the SAAF from a ciona intestinalis by using a JMS-S3000 SpiralTOF™ equipped with the optional TOF-TOF analyzer to generate a high energy CID product ion spectrum for this compound.

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SpiralTOF-TOF Analysis of Bovine Serum Albumin
SpiralTOF-TOF Analysis of Bovine Serum Albumin

In a previous application note, we reported the analysis of bovine serum albumin (BSA) by Spiral and Linear modes. In this work, we show the analytical result for BSA by using the JMS-S3000 "SpiralTOF" with the TOF-TOF option.

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Detection of molecular ions of fluorine compounds by GC/FI-TOFMS
Detection of molecular ions of fluorine compounds by GC/FI-TOFMS

This application note shows that FI is a suitable technique to determine the molecular weight of compounds which are not producing molecular ions in EI or CI.

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