Analytical Instrument Documents

In this Application Note we present an example of hot to efficiently obtain a set of full 13C & 1H assignments of Kanamycin-A disulfate (MW ~880 Da), using only 1 mg of the antibiotic and four basic NMR experiments and utilizing JEOL's new nitrogen-cooled SuperCool MARVEL probe.

Nitroxide radicals exhibit anisotropic g-values and hyperfine interactions depending on their molecular orientation relative to the magnetic field. However, in aqueous solutions, molecular motion is sufficiently fast on the ESR timescale to result in effective averaging of this orientational anisotropy. Consequently, motional narrowing occurs, as all molecules experience an equivalent magnetic field, leading to sharp ESR signals.

Sensitivity correction typically involves calibration using a standard sample with a known spin concentration, normalization with a Mn marker, normalization by sample mass, volume, or amount, and temperature correction based on the Boltzmann distribution.

Pyrazines are known as key aroma compounds that characterize the roasted aroma of Hojicha, and in recent years, they have attracted attention for their potential health benefits. Thermal desorption (TD)-GC-MS is an analytical method used for analyzing food aroma components. Although the method enables highly sensitive and comprehensive analysis of aroma compounds in samples, searching for specific target compounds among the many detected components can be time-consuming.

In this study, we report a comparative analysis of dioxin analysis by hydrogen carrier gas with helium carrier gas using GC/MS/MS “JMS-TQ4000GC UltraQuad™ TQ” (Figure 1).

We measured commercially available Japanese curry roux, which is composed of various ingredients and extracts, such as spices, meat, and vegetables, and is thought to have a complex aroma component. Volatile components extracted by headspace solid phase microextraction (HS-SPME) were measured using GCxGC-TOFMS, and the results of analysis using msFineAnalysis AI are reported.

The qualitative analysis software msFineAnalysis AI enables automatic structure analysis of unknown compounds not registered in libraries through EI/SI integrated analysis and AI structure analysis. Ver. 3 newly supports comprehensive 2D GC (GCxGC) data analysis.

This MSTips introduces the usefulness of AI structure analysis using polymer pyrolysis analysis. Figure 1 shows a schematic diagram of pyrolysis (Py)-GCxGC-MS.

This MSTips introduces technique for comparing samples and identifying differential peaks using 2D chromatograms, using pyrolysis oil analysis. Pyrolysis oil is derived from discarded plastics and rubber products. It is used as an alternative fuel to petroleum and as a raw material for recycled plastics, making it one of the key technologies contributing to the circular economy.

In this MSTips, non-targeted measurements of metabolites in mouse feces were performed using GC-TOFMS, followed by data analysis using msFineAnalysis AI.